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Characterization of biocide-tolerant bacteria isolated from artisanal cheeses

Grant number: 19/03176-9
Support Opportunities:Scholarships abroad - Research Internship - Master's degree
Effective date (Start): April 02, 2019
Effective date (End): May 29, 2019
Field of knowledge:Agronomical Sciences - Food Science and Technology - Food Science
Principal Investigator:Bernadette Dora Gombossy de Melo Franco
Grantee:Josisleine Recalde Allaion Ferreira
Supervisor: María José Grande Burgos
Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Research place: Universidad de Jaén, Spain  
Associated to the scholarship:18/02630-5 - Microbiological characterization of ripened Minas artisanal cheese marketed in São Paulo: safety, hygiene and microbial diversity, BP.MS


Biocides, because they have antiseptic and/or disinfectant action, have become popular for the disinfection of surfaces and materials in the food industry. The increased use of biocides is of concern because it can induce cross-resistance to antibiotics. In Brazil, the legislation for supervision of production and marketing of artisanal cheeses is complex and has numerous gaps, and these products can become an important public health issue, due to contamination with pathogenic bacteria. As extensive use of biocides can lead to resistance to antibiotics, this BEPE project aims to determine the biocide tolerance of staphylococci isolated from artisanal cheeses, in order to evaluate their possible contribution in the transmission of antimicrobial resistance in the cheese production chain. The phenotypic profiles will be analysed by determination of the Minimum Inhibitory Concentrations (MIC) for benzalkonium chloride, cetrimide, hexadecyl pyridinium chloride, triclosan and hexachlorophene using the microdilution method in broth in microtiter plates and the genotypic profiles will be determined by Polymerase Chain Reaction (PCR) for the qacA/B and smr (qacC) genes. The statistical significance of the correlations between biocide tolerance levels and presence of biocide tolerant genes will be analyzed with the SPSS statistical program. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ALLAION, JOSISLEINE RECALDE; BARRIONUEVO, KARINA GHOUGASSIAN; GOMBOSSY DE MELO FRANCO, BERNADETTE DORA. ssessing the Microbiological Safety Parameters of Minas Artisanal Cheese Samples in Retail Environments in Sao Paulo, Brazi. APPLIED SCIENCES-BASEL, v. 11, n. 19, . (13/07914-8, 19/03176-9, 18/02630-5)
ALLAION, JOSISLEINE RECALDE; BARRIONUEVO, KARINA GHOUGASSIAN; GRANDE BURGOS, MARIA JOSE; GALVEZ, ANTONIO; FRANCO, BERNADETTE DORA GOMBOSSY DE MELO. taphylococcus aureus from Minas Artisanal Cheeses: Biocide Tolerance, Antibiotic Resistance and Enterotoxin Gene. PPLIED SCIENCES-BASE, v. 12, n. 3, . (19/03176-9, 18/02630-5, 13/07914-8, 18/17862-9)

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