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Obtaining symbiotic microcapsules of xylo-oligosaccharides from sugarcane straw

Grant number: 18/14223-5
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): March 01, 2019
Effective date (End): February 29, 2020
Field of knowledge:Agronomical Sciences - Food Science and Technology - Food Engineering
Principal Investigator:Rosana Goldbeck Coelho
Grantee:Manoela Martins
Host Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated research grant:15/20630-4 - Biorefinery development integrated to a bioethanol sugar cane plant with zero CO2 emission: routes to convert renewable resources to bio-products and bio-electricity, AP.TEM


Agroindustrial waste, besides being abundant, is the source of several components of industrial interest, which can be exploited and used for the production of bioactive molecules of high value in an inexpensive, renewable and sustainable way. The sugarcane straw generated during cane processing is rich in xylan, which, after extraction, is susceptible to the action of the xylanase enzymes for the production of xylo-oligosaccharides, which shows probiotic potential, improving the viability of prebiotic microorganisms in food. Thus, this present work seeks to explore the application of sugarcane straw to the production of xylo-oligosaccharides through the action of the xylanase enzyme, produced from the heterologous expression of Cryptococcus flavescens isolated from the Brazilian Biome, and to study the symbiotic association of these components and probiotic bacteria, aiming the production of symbiotic microparticles. The digestibility of the microcapsules obtained will be analyzed by "in vitro" tests, and the resistance to the hydrolysis by enzymes of the digestive tract, as well as the stimulation of the growth of the probiotic bacteria, will be evaluated. It is expected at the end of the project that the microcapsules produced from xylo-oligosaccharides from sugarcane straw present symbiotic potential so they can be applied industrially as functional ingredients in food products. We would also like to mention that this project is directly integrated to FAPESP Thematic Project (2015 / 20630-4) entitled "Integrated Development of Biorefinery and Sugarcane Bioethanol Plant with Zero CO2 Emission: Routes to convert renewable resources into bio-products and bio-electricity", in which we are inserted. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MARTINS, MANOELA; SATO, ANA CARLA KAWAZOE; OGINO, KENJI; GOLDBECK, ROSANA. valuating the addition of xylooligosaccharides into alginate-gelatin hydrogel. Food Research International, v. 147, . (18/14223-5, 19/08542-3, 15/20630-4)
BARBOSA, FERNANDO CESAR; MARTINS, MANOELA; BRENELLI, LIVIA BEATRIZ; FERRARI, FELIPE AUGUSTO; SOARES FORTE, MARCUS BRUNO; RABELO, SARITA CANDIDA; FRANCO, TELMA TEIXEIRA; GOLDBECK, ROSANA. Screening of potential endoglucanases, hydrolysis conditions and different sugarcane straws pretreatments for cello-oligosaccharides production. Bioresource Technology, v. 316, p. 8-pg., . (18/14223-5, 19/08542-3, 17/15477-8, 15/50612-8, 19/19976-4)
MARTINS, MANOELA; AVILA, PATRICIA FELIX; PAIM DE ANDRADE, CRISTIANE CONTE; GOLDBECK, ROSANA. Synergic recombinant enzyme association to optimize xylo-oligosaccharides production from agricultural waste. BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, v. 28, p. 8-pg., . (15/20630-4, 18/14223-5, 15/50612-8, 19/08542-3)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
MARTINS, Manoela. Obtaining synbiotic microparticles from xylo-oligosaccharides from sugarcane straw. 2020. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia de Alimentos Campinas, SP.

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Filed patent(s) as a result of this research project

PROCESSO DE PRODUÇÃO DE MICROPARTÍCULAS SIMBIÓTICAS E SEU USO PARA PREPARAÇÃO DE PRODUTOS ALIMENTÍCIOS PCT/BR2021/050248 - Universidade Estadual de Campinas (UNICAMP) . Ana Carla Kawazoe Sato ; Rosana Goldbeck ; Manoela Martins ; Cristiane Conte Paim De Andrade - June 2021, 09