Improving method of drug-delivery system with ionic-cyclodextrin and their inclusion complexes with antimicrobial agent into the polymeric multilayers

Abstract

Antimicrobial coatings to dental implant materials devices are considered a required challenge for peri-implantitis treatment. In particular, antimicrobial surfaces could disturb the onset of pathogenic biofilm formation and avoid infection progression with posterior implant loss. Ideally, the tissue surrounding the implant should inhibit apical epithelial migration and provide a biological seal between the material and soft tissues to protect the implant against mechanical forces and microorganisms. However, the fact of the oral mucosa implant-interface does not totally isolate the internal environment may facilitate pathogens invasion to the body. Inspired by previous knowledge, in this project we will focus on the emerging options to dental implants-related infection prevention and treatment, from a biomaterial perspective, considering biomaterial-system outcomes acquired in our previous study. The proposed research is innovative because we will develop a novel non-cytotoxic antimicrobial coating to implant substrates. Having conducted some preliminary research, we will now be in a position to deep down and improve the method of drug incorporation into the multilayers and the drug concentration released over time. A detailed characterization of anionic beta cyclodextrins (anionic ²-CDs), obtained by a single step polycondensation reaction between epichlorohydrin and chloroacetic acid, will be performed by nuclear magnetic resonance (NMR). Drug release concentration will be confirmed under neutral, acidic and alkaline pH, mimicking healthy and diseased/inflammatory environments, respectively by high-performance liquid chromatography (HPLC). Sustained antibacterial effect of drugs released from LbL system will be confirmed against a Staphylococcus aureus, a potential pathogen disclosing a strong capacity to efficiently attach onto titanium substrates and influencing the biofilm behavior.