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Nanostructured films applied in microfluidic biosensors to mastitis detection

Grant number: 18/18953-8
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): March 01, 2019
Effective date (End): February 28, 2023
Field of knowledge:Engineering - Materials and Metallurgical Engineering - Nonmetallic Materials
Principal researcher:Luiz Henrique Capparelli Mattoso
Grantee:Andrey Coatrini Soares
Home Institution: Embrapa Instrumentação Agropecuária. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). Ministério da Agricultura, Pecuária e Abastecimento (Brasil). São Carlos , SP, Brazil
Associated research grant:18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis, AP.TEM

Abstract

The study and development of biosensors has focused on the use of microfluidic devices, functionalized with biomolecules, which allows miniaturization, low cost and quick results. In this work, biosensors will be developed from microfluidic devices functionalized in different molecular architectures for mastitis detection in milk samples. The first part of the project provides for the molecular architectures fabrication using self-assembly techniques, combining the properties of chitosan, chondroitin sulfate and sericin, and the characterization of these nanostructures. From the optimization of manufacturing conditions, the better architectures will be functionalized Anti-S-aureus antibodies to detect Staphylococcus aureus. Electrical impedance spectroscopy will be used to obtain calibration curves, which will be used in the establishment of adsorption models in biosensors and analyzes of milk samples contaminated with bacterias, obtained from Embrapa Gado de Leite. In addition to detection, selectivity and false positive biosensors analysis will be held through information visualization maps, allowing the optimization of the best conditions and parameters for specific sensing units. Finally, morphology and chemical interactions studies are performed through PM-IRRAS spectroscopy, and AFM analysis. The combination of characterization and detection techniques will optimize sensitivity and selectivity performance for mastitis detection and analysis of milk quality.

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Scientific publications (14)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SOARES, JULIANA COATRINI; MELENDEZ, MATIAS ELISEO; SOARES, ANDREY COATRINI; ARANTES, LIDIA MARIA REBOLHO BATISTA; RODRIGUES, VALQUIRIA DA CRUZ; CARVALHO, ANDRE LOPES; REIS, RUI MANUEL; MATTOSO, LUIZ HENRIQUE C.; OLIVEIRA, JR., OSVALDO N.. Detection of HPV16 in cell lines deriving from cervical and head and neck cancer using a genosensor made with a DNA probe on a layer-by-layer matrix. MATERIALS CHEMISTRY FRONTIERS, v. 4, n. 11, p. 3258-3266, . (18/18953-8, 18/22214-6)
MAIA ROCHA NETO, JOAO BATISTA; SOARES, ANDREY COATRINI; BATAGLIOLI, ROGERIO APARECIDO; CARR, OLIVIA; RODRIGUES COSTA, CARLOS ALBERTO; OLIVEIRA JR, OSVALDO N.; BEPPU, MARISA MASUMI; CARVALHO, HERNANDES F.. Polysaccharide Multilayer Films in Sensors for Detecting Prostate Tumor Cells Based on Hyaluronan-CD44 Interactions. CELLS, v. 9, n. 6, . (18/22214-6, 18/20560-4, 18/18953-8, 16/10193-9, 14/50938-8)
SOARES, ANDREY COATRINI; SOARES, JULIANA COATRINI; PASCHOALIN, RAFAELLA TAKEHARA; RODRIGUES, VALQUIRIA CRUZ; MELENDEZ, MATIAS ELISEO; REIS, RUI M.; CARVALHO, ANDRE LOPES; CAPPARELLI MATTOSO, LUIZ HENRIQUE; OLIVEIRA JR, OSVALDO N.. Immunosensors containing solution blow spun fibers of poly(lactic acid) to detect p53 biomarker. Materials Science & Engineering C-Materials for Biological Applications, v. 115, . (18/18953-8, 13/14262-7, 17/18725-2)
SOARES, JULIANA COATRINI; SOARES, ANDREY COATRINI; RODRIGUES, VALQUIRIA CRUZ; MELENDEZ, MATIAS ELISEO; SANTOS, ALEXANDRE CESAR; FARIA, ELINEY FERREIRA; REIS, RUI M.; CARVALHO, ANDRE LOPES; OLIVEIRA, JR., OSVALDO N.. Detection of the Prostate Cancer Biomarker PCA3 with Electrochemical and Impedance-Based Biosensors. ACS APPLIED MATERIALS & INTERFACES, v. 11, n. 50, p. 46645-46650, . (18/18953-8, 13/14262-7)
PICCOLI, JULIA P.; SOARES, ANDREY C.; OLIVEIRA, JR., OSVALDO N.; CILLI, EDUARDO M.. Nanostructured functional peptide films and their application in C-reactive protein immunosensors. BIOELECTROCHEMISTRY, v. 138, . (18/22214-6, 13/07600-3, 18/18953-8, 17/24839-0, 18/18231-2)
SOARES, JULIANA C.; SOARES, ANDREY C.; ANGELIM, MONARA KAELLE S. C.; PROENCA-MODENA, JOSE LUIZ; MORAES-VIEIRA, PEDRO M.; MATTOSO, LUIZ H. C.; OLIVEIRA JR, OSVALDO N.. iagnostics of SARS-CoV-2 infection using electrical impedance spectroscopy with an immunosensor to detect the spike protei. Talanta, v. 239, . (18/22214-6, 20/08744-2, 18/18953-8, 20/04579-7)
SOARES, JULIANA COATRINI; SOARES, ANDREY COATRINI; RODRIGUES, VALQUIRIA CRUZ; OITICICA, PEDRO RAMON ALMEIDA; RAYMUNDO-PEREIRA, PAULO AUGUSTO; BOTT-NETO, JOSE LUIZ; BUSCAGLIA, LORENZO A.; DE CASTRO, LUCAS DANIEL CHIBA; RIBAS, LUCAS C.; SCABINI, LEONARDO; et al. Detection of a SARS-CoV-2 sequence with genosensors using data analysis based on information visualization and machine learning techniques. MATERIALS CHEMISTRY FRONTIERS, . (18/22214-6, 18/19750-3, 18/18953-8, 16/01919-6, 19/13514-9, 14/50867-3, 20/02938-0, 19/00101-8, 19/07811-0, 16/23763-8)
NETO, MARIO POPOLIN; SOARES, ANDREY COATRINI; OLIVEIRA JR, OSVALDO N.; PAULOVICH, V, FERNANDO. Machine Learning Used to Create a Multidimensional Calibration Space for Sensing and Biosensing Data. BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, v. 94, n. 5, p. 1553-1562, . (18/18953-8, 18/22214-6)
SOARES, ANDREY COATRINI; SOARES, JULIANA COATRINI; RODRIGUES, VALQUIRIA CRUZ; OLIVEIRA, JR., OSVALDO N.; CAPPARELLI MATTOSO, LUIZ HENRIQUE. Controlled molecular architectures in microfluidic immunosensors for detecting Staphylococcus aureus. ANALYST, v. 145, n. 18, p. 6014-6023, . (18/22214-6, 18/18953-8)
RODRIGUES, VALQUIRIA C.; SOARES, JULIANA C.; SOARES, ANDREY C.; BRAZ, DANIEL C.; MELENDEZ, MATIAS ELISEO; RIBAS, LUCAS C.; SCABINI, LEONARDO F. S.; BRUNO, ODEMIR M.; CARVALHO, ANDRE LOPES; REIS, RUI MANUEL; et al. Electrochemical and optical detection and machine learning applied to images of genosensors for diagnosis of prostate cancer with the biomarker PCA3. Talanta, v. 222, . (19/07811-0, 18/22214-6, 18/18953-8, 16/23763-8)
LEITE, LILIANE S. F.; BILATTO, STANLEY; PASCHOALIN, RAFAELLA T.; SOARES, ANDREY C.; MOREIRA, V, FRANCYS K.; OLIVEIRA, JR., OSVALDO N.; MATTOSO, LUIZ H. C.; BRAS, JULIEN. Eco-friendly gelatin films with rosin-grafted cellulose nanocrystals for antimicrobial packaging. International Journal of Biological Macromolecules, v. 165, n. B, p. 2974-2983, . (17/18725-2, 18/18953-8, 18/10899-4, 18/22214-6, 18/00278-2)
GRABOSKI, ADRIANA M.; ZAKRZEVSKI, CLAUDIO A.; SHIMIZU, FLAVIO M.; PASCHOALIN, RAFAELLA T.; SOARES, ANDREY C.; STEFFENS, JULIANA; PAROUL, NATALIA; STEFFENS, CLARICE. Electronic Nose Based on Carbon Nanocomposite Sensors for Clove Essential Oil Detection. ACS SENSORS, v. 5, n. 6, p. 1814-1821, . (18/18953-8, 17/18725-2)
SOARES, JULIANA COATRINI; SOARES, ANDREY COATRINI; RODRIGUES, VALQUIRIA CRUZ; OITICICA, PEDRO RAMON ALMEIDA; RAYMUNDO-PEREIRA, PAULO AUGUSTO; BOTT-NETO, JOSE LUIZ; BUSCAGLIA, LORENZO A.; DE CASTRO, LUCAS DANIEL CHIBA; RIBAS, LUCAS C.; SCABINI, LEONARDO; et al. Detection of a SARS-CoV-2 sequence with genosensors using data analysis based on information visualization and machine learning techniques. MATERIALS CHEMISTRY FRONTIERS, v. 5, n. 15, p. 5658-5670, . (19/07811-0, 18/19750-3, 20/02938-0, 16/01919-6, 18/18953-8, 19/13514-9, 14/50867-3, 16/23763-8, 18/22214-6, 19/00101-8)
SOARES, JULIANA COATRINI; SOARES, ANDREY COATRINI; POPOLIN-NETO, MARIO; PAULOVICH, FERNANDO VIEIRA; OLIVEIRA JR, OSVALDO N.; CAPARELLI MATTOSO, LUIZ HENRIQUE. etection of Staphylococcus aureus in milk samples using impedance spectroscopy and data processing with information visualization techniques and multidimensional calibration space. SENSORS AND ACTUATORS REPORTS, v. 4, . (18/22214-6, 18/18953-8)

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