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High throughput biocompatible SPME coatings for metabolomic analyses

Grant number: 18/25729-7
Support Opportunities:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): July 01, 2019
Effective date (End): December 31, 2019
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
Principal Investigator:Fabio Augusto
Grantee:Joao Raul Belinato de Souza
Supervisor: Janusz Pawliszyn
Host Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Research place: University of Waterloo, Canada  
Associated to the scholarship:16/20547-2 - Characterization of the metabolome of anti-phytopatogen microorganisms by Multidimensional Gas Chromatography and Simple / Tandem Mass Spectrometry (GC×GC-QMS and GC×GC-MS/MS), BP.DR


This doctorate research internship abroad (BEPE-DR) project is a part of the National Institute of Bioanalytical Science and Technology (INCTBio) and FAPESP doctorate project (Proc. 2016 / 20547-2). This project aims to study high throughput Thin-Film SPME (TF-SPME) devices for global metabolic profiling of biological samples. These systems will be used as model for targeted and untargeted metabolomics studies. TF-SPME devices will be prepared and characterized according to standard procedures found in literature. TF-SPME will be initially used to explore non-volatile metabolome of biological samples (untargeted and targeted) using LC-MS systems. After that, volatile metabolome from biological samples' systems will be evaluated using GCxGC-TOFMS platforms. All experiments will be done at the University of Waterloo, Canada, under supervision of Professor Janusz Pawliszyn.

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Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BELINATO, JOAO RAUL; ACQUARO, JR., VINICIUS RICARDO; DIAS CAPPELINI, LUCIANA TERESA; AUGUSTO, FABIO. New prospects and problems in sample preparation methods for microbiome analysis. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, v. 143, . (18/25729-7, 17/08038-8, 18/03956-1, 16/20547-2, 14/07037-0)
POLLO, BRENO JORGE; TEIXEIRA, CARLOS ALBERTO; BELINATO, JOAO RAUL; FURLAN, MAYRA FONTES; DE MATOS CUNHA, ISABELA CRISTINA; VAZ, CAROLINE ROCHA; VOLPATO, GUSTAVO VERONEZI; AUGUSTO, FABIO. Chemometrics, Comprehensive Two-Dimensional gas chromatography and ``omics{''} sciences: Basic tools and recent applications. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, v. 134, . (19/12556-0, 14/50867-3, 18/25729-7)
GRANDY, JONATHAN J.; MURTADA, KHALED; BELINATO, JOAO RAUL; ORTIZ SUAREZ, PAOLA ALEJANDRA; PAWLISZYN, JANUSZ. Development and validation of an improved, thin film solid phase microextraction based, standard gas generating vial for the repeatable generation of gaseous standards. Journal of Chromatography A, v. 1632, . (18/25729-7)
BELINATO, JOAO RAUL; GRANDY, JONATHAN J.; KHALED, ABIR; SUAREZ, PAOLA ALEJANDRA ORTIZ; PAWLISZYN, JANUSZ. Overcoming matrix effects in the analysis of pyrethroids in honey by a fully automated direct immersion solid-phase microextraction method using a matrix-compatible fiber. Food Chemistry, v. 340, . (18/25729-7, 16/20547-2)

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