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In vitro evaluation of surface loss and color change of bovine enamel submitted to erosive/abrasive challenges with different whitening and desensitizing dentifrices

Grant number: 18/09335-9
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): December 01, 2018
Effective date (End): January 31, 2020
Field of knowledge:Health Sciences - Dentistry
Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Taís Scaramucci Forlin
Grantee:Leonardo Custódio de Lima
Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil


This study will analyze surface loss and color change after an erosive/abrasive cycling performed with different dentifrices with whitening, and desensitizing or only whitening claims, in comparison to their non-whitening versions, when these are available. For the surface loss analysis, 130 enamel fragments from bovine incisors (4 mm×4 mm×2 mm) will be used. They will be embedded in acrylic resin, ground flat and polished. For the color change analysis, 130 enamel fragments from bovine incisors (6 mm×6 mm×2mm) will be used. The specimens will be randomly allocated into 13 experimental groups, according to the treatments: 1. Distilled water (control); 2. Sensodyne Repair & Protect - Regular; 3. Sensodyne Repair & Protect - Branqueador; 4. Colgate Sensitive Original; 5. Colgate Sensitive Branqueador; 6. Colgate Sensitive Pró-Alívio - Regular; 7. Colgate Sensitive Pró-Alívio - Branqueador; 8. Colgate Total 12 - Regular; 9. Colgate Total 12 - Branqueador; 10. Sensodyne True White; 11. Curaprox Black is White; 12. Oral-B 3D white perfection and 13. Erosive cycling only. Specimens will be submitted to an erosive/abrasive cycling consisting of 2 min immersion in 1% citric acid solution (pH~2,4), followed by artificial saliva exposure for 60min, 6×/day, for 5 days. Brushing will be performed 2×/day, 30 min after the first and last erosive challenges, with a slurry of one of the dentifrices above mentioned and artificial saliva, in 1:3 ratio. At the end of the cycle, enamel surface loss (in ¼m) will be determined with an optical profilometer, and the color change will be determined in an ultraviolet visible light spectrophotometer, according to the system parameters CIELAB (Comission Internationale de I clairage L*, a*, b*).

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LIMA, LEONARDO CUSTODIO DE; VIANA, ITALLO EMIDIO LIRA; PAZ, SARA LAIS PEREIRA DA; BEZERRA, SAVIO JOSE CARDOSO; JOAO-SOUZA, SAMIRA HELENA; CARVALHO, THIAGO SAADS; SCARAMUCCI, TAIS. Role of desensitizing/whitening dentifrices in enamel wear. Journal of Dentistry, v. 99, . (18/07284-8, 18/09335-9, 17/14691-6)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
LIMA, Leonardo Custódio de. In vitro evaluation of surface loss and color change of enamel submitted to erosive/abrasive challenges with different whitening and desensitizing dentifrices. 2020. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Odontologia (FO/SDO) São Paulo.

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