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Mass spectrometry analysis of a new phosphodiesterase from Crotalus durissus collilineatus venom

Grant number: 18/21233-7
Support Opportunities:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): March 01, 2019
Effective date (End): August 31, 2019
Field of knowledge:Health Sciences - Pharmacy - Toxicological Analysis
Principal Investigator:Eliane Candiani Arantes Braga
Grantee:Isadora Sousa de Oliveira
Supervisor: Loic Quinton
Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Research place: Université de Liège (ULg), Belgium  
Associated to the scholarship:17/03580-9 - Biochemical, structural and functional evaluation of a phosphodiesterase from Crotalus durissus collilineatus venom, BP.DR


Snake venoms present a wide range of components able to induce various pathological symptoms in victims of envenoming, which is part of the neglected circumstances by WHO and is a serious public health problem in Brazil. Snakes of Crotalus durissus species represent Crotalus genus in the national territory. Crotalus durissus collilineatus venom presents a great complexity of protein components, which contribute to the toxic effects observed in the crotalic accident. However, due to its complexity, many components have not yet been isolated, as phosphodiesterase (PDE). These enzymes are part of the nucleases, such as DNAse and RNAse, and have a high molecular mass (90 ~160 kDa), being found in monomeric or dimeric forms. PDEs are responsible for the cleavage of phosphodiester bonds of nucleic acids, ATP, ADP, NAD, NGD, cAMP and cGMP, being able to interfere in physiological or pathological processes, which make them therapeutic targets of various diseases. Their role in the snakebite envenoming is still not fully elucidated, but it is known that PDEs are capable to leading to hypotension and locomotor depression of the prey and they are also capable of inhibiting platelet aggregation in vitro. Therefore, the objectives of this study are to determine the molecular mass of PDE from C. d. collilineatus venom, its full amino acid sequence, using a multi enzymatic limited digestion, and its glycosylation pattern, showing glycosylation sites and carbohydrate composition. All of these assays will be performed by mass spectrometry techniques. In this way, the present study becomes relevant because will analyze structurally a toxin that is important for snakebite envenoming, but still little studied, increasing the knowledge about this protein and its enzymatic class.

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Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE OLIVEIRA, ISADORA SOUSA; PUCCA, MANUELA BERTO; WIEZEL, GISELE ADRIANO; CARDOSO, IARA AIME; FIGUEIREDO BORDON, KARLA DE CASTRO; SARTIM, MARCO AURELIO; KALOGEROPOULOS, KONSTANTINOS; AHMADI, SHIRIN; BAIWIR, DOMINIQUE; NONATO, MARIA CRISTINA; et al. Unraveling the structure and function of CdcPDE: A novel phosphodiesterase from Crotalus durissus collilineatus snake venom. International Journal of Biological Macromolecules, v. 178, p. 180-192, . (17/00586-6, 17/03580-9, 19/10173-6, 11/23236-4, 18/21233-7)
FIGUEIREDO BORDON, KARLA DE CASTRO; COLOGNA, CAMILA TAKENO; FORNARI-BALDO, ELISA CORREA; PINHEIRO-JUNIOR, ERNESTO LOPES; CERNI, FELIPE AUGUSTO; AMORIM, FERNANDA GOBBI; PINO ANJOLETTE, FERNANDO ANTONIO; CORDEIRO, FRANCIELLE ALMEIDA; WIEZEL, GISELE ADRIANO; CARDOSO, IARA AIME; et al. From Animal Poisons and Venoms to Medicines: Achievements, Challenges and Perspectives in Drug Discovery. FRONTIERS IN PHARMACOLOGY, v. 11, . (17/14035-1, 17/00586-6, 18/14158-9, 19/10173-6, 18/21233-7, 17/04724-4, 13/26619-7, 16/04761-4, 17/03580-9, 13/26200-6)
OLIVEIRA, ISADORA S.; FERREIRA, ISABELA G.; ALEXANDRE-SILVA, GABRIEL M.; CERNI, FELIPE A.; CREMONEZ, CAROLINE M.; ARANTES, ELIANE C.; ZOTTICH, UMBERTO; PUCCA, MANUELA B.. Scorpion toxins targeting Kv1.3 channels: insights into immunosuppression. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 25, . (18/21233-7, 17/14035-1, 18/14158-9, 17/03580-9)
ABREU, CAIO B.; BORDON, KARLA C. F.; CERNI, FELIPE A.; OLIVEIRA, ISADORA S.; BALENZUELA, CARLA; ALEXANDRE-SILVA, GABRIEL M.; ZOCCAL, KARINA F.; REIS, MOUZARLLEM B.; WIEZEL, GISELE A.; PEIGNEUR, STEVE; et al. Pioneering Study onRhopalurus crassicaudaScorpion Venom: Isolation and Characterization of the Major Toxin and Hyaluronidase. FRONTIERS IN IMMUNOLOGY, v. 11, . (17/00586-6, 16/04761-4, 17/04724-4, 17/03580-9, 19/10173-6, 17/14035-1, 18/21233-7)
PUCCA, MANUELA B.; CERNI, FELIPE A.; OLIVEIRA, ISADORA S.; JENKINS, TIMOTHY P.; ARGEMI, LIDIA; SORENSEN, CHRISTOFFER V.; AHMADI, SHIRIN; BARBOSA, JOSE E.; LAUSTSEN, ANDREAS H.. Bee Updated: Current Knowledge on Bee Venom and Bee Envenoming Therapy. FRONTIERS IN IMMUNOLOGY, v. 10, . (18/14158-9, 18/21233-7, 17/04724-4, 17/14035-1, 17/03580-9)

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