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Decellularization of extracellular matrix of canine mammary solid carcinoma

Grant number: 18/18855-6
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): November 01, 2018
Effective date (End): October 31, 2019
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Pathology
Principal researcher:Maria Angelica Miglino
Grantee:Jessica Batista Nobre
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Cancer is characterized by abnormal physiological changes in a cell of the body, which modifies its functionality and especially its growth and development that happens to be disorderly and progressive. Among tumors in bitches, the most common are mammary tumors, with a high incidence with a high mortality rate. The malignant mammary tumors have great potential to undergo a process of tumor invasion and migration of tumor cells, and for this the extracellular matrix plays a fundamental role. The decellularization of its extracellular matrix allows the retention of most of its natural properties and its three-dimensional shape, essential for the study of the components present in its matrix. Thus, the objective of this work is to induce the formation of canine mammary solid carcinoma through tumor cells previously established in nude immunosuppressed mice, analyzing their growth and components present in their extracellular matrix. For this purpose, solid canine mammary carcinoma cells (TCM12) will be cultured and 1x106 cells in 5 immunosuppressed mice applied. After tumor formation, these animals will be euthanized and the tumors will be collected and their extracellular matrix analyzed. For the analysis of the extracellular matrix will be used techniques of light microscopy, transmission electron microscopy, immunohistochemical analysis and DNA quantification.

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