Disturbances of oligodendrocytes and myelin integrity are observed in certain disorders, such as schizophrenia. In this context, toxic models of demyelination are used to understand the mechanisms involved in demyelination, remyelination and oligodendrocyte alterations. On the other hand, proteomic approaches are important for the understanding of processes involved in neurological disorders, through the elucidation of biochemical bases, and identification of differentially expressed pathways and proteins, in certain cerebral disorders. In the present work, the cuprizone-induced demyelination model will be used in human oligodendrocyte cell cultures (lineage MO3.13) to test the effects of antipsychotics (clozapine and haloperidol), cannabidiol and benztropine, in terms of their potential neuroprotective effects. After the treatments mentioned above, the proteome will be extracted and digested for analysis by nano-chromatography coupled to mass spectrometry. Thereby, this project aims to investigate the proteins and pathways involved in the demyelination, remyelination and proliferation of oligodendrocytes, in schizophrenia model. In this context, the present study will contribute to the understanding of the molecular biochemical mechanisms involved in the action of these drugs on oligodendrocytes, and their possible implications on the pathophysiology and treatment of schizophrenia.
News published in Agência FAPESP Newsletter about the scholarship: