Antimicrobial and biocompatibility potentials of hexamethyldisiloxane films incorporated with chlorhexidine on titanium surface using a co-infected in vitro reconstituted gingival oral epithelium model
A stable connection between the titanium implant surface/abutment and periimplant soft tissues is an important approach for the long-term success of dental implant treatment. Thus, such surface should minimize the bacterial colonization while allowing a good adhesion of the oral tissues. Therefore, the objective of this study is to evaluate the antimicrobial and biocompatibility potentials of hexamethyldisiloxane films incorporated with and without (control) chlorhexidine (CHX) onto commercially pure titanium (cpTi) surface on a co-infected in vitro reconstituted oral epithelium model. Machined (M) surface will also be used as control. The development and characterization of such surface and its in vitro antimicrobial and biocompatibility potentials were tested in Brazil in the PhD scholarship project. However, we were not able to conduct co-infection model that represents a more realistic clinical scenario. Therefore, for the co-infection of an in vitro reconstituted oral epithelium assay, immunofluorescence imaging, metabolic activity, histology, cytotoxicity test (LDH release), viability test (MTT assay) and immunohistochemistry (Ki67 expression) will be conducted. Quantitative data will be statistically analyzed at a significant level of 5%. The number of samples for each assay will be determined by a pilot study.
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