Advanced search
Start date
Betweenand

Uncovering new regulators of DAF-16/FOXO expression

Grant number: 18/07703-0
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): August 01, 2018
Effective date (End): February 29, 2020
Field of knowledge:Biological Sciences - Genetics - Animal Genetics
Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Marcelo Alves da Silva Mori
Grantee:Sweta Sarmah
Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated research grant:17/01184-9 - CAMeLEOm: cross-species analysis of metabolic, lifespan effects and omics of dietary restriction mimetics, AP.TEM

Abstract

Insulin/IGF-1-like signaling (IIS) is central to growth and metabolism and has a conserved role in aging. In C. elegans, key components of this pathway are the insulin/IGF-1 receptor ortholog DAF-2 and the FoxO transcription factor ortholog DAF-16. Mutations in the daf-2 gene make worms live twice as long, and this phenomenon is entirely dependent on DAF-16 activation. Similarly, other conditions that extend lifespan in C. elegans, such as germline ablation and dietary restriction, require DAF-16. Preliminary data from our lab identified new potential regulators of DAF-16. In a RNAi screen, we found adr-1, nrde-1, wago-1, hda-1, and set-25 as positive regulators of a DAF-16 reporter transgene expression in germline-less worms. These genes are involved in histone modification and/or small RNA-mediated silencing pathways, and some participate in transgenerational inheritance. This suggests that DAF-16 and aging may be subject to epigenetic regulation through such previously unidentified mechanisms. In this project we aim to confirm by several methods whether DAF-16 is indeed controlled by these proteins and how. We also plan to test whether they participate in DAF-16-mediated lifespan regulation. With that, we hope to describe new mechanisms of aging, some of which could be epigenetically inherited and evolutionarily conserved.

News published in Agência FAPESP Newsletter about the scholarship:
Articles published in other media outlets (0 total):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Please report errors in scientific publications list using this form.