The insulin topical use in formula gel vs cream in repair of tissue diabetic mice

Abstract

Tissue repair is a biological, complex and dynamic process and when associated with Diabetes Mellitus has negative modulators of insulin signaling that trigger a consequent delay in wound healing. Studies show that Insulin modulates the inflammatory phase of the healing process, stimulates the proliferation of fibroblasts and accelerates other stages of the healing process, improving the quality of scar tissue. The objective of this study will be to compare the effect of topical Insulin gel treatment with insulin cream on the healing process of diabetic mice. Materials and methods: the study will be composed of three groups: 1º. Diabetic animals with wounds treated with insulin cream; 2º. Diabetic animals with wounds treated with insulin gel and 3rd. Diabetic animals with wounds treated with saline 0.9%. Diabetes will be induced with streptozotocin (50mg / kg / 5 days / intraperitoneal). Under anesthesia, the wound in the dorsal region will be made with 1 cm2 mold, being treated daily and removed on days 4 and 10 for further analysis. The healing process will be evaluated macroscopically by means of photographs at days zero, 2, 4, 7 and 10 post-injury and microscopically on hematoxylin & Eosin histology, and on the labeling of proteins of the insulin signaling pathway by immunohistochemistry. We will use the Image J software, the Student T Test and one-way ANOVA for statistical analysis. The level of significance will be p <0.05.