The antinociceptive effect of botulinum toxin type A (BoNT-A) is, in part, due to the peripheral inhibition of pain mediators. On the other hand, authors indicate a central involvement of the neurotoxin, with a possible interaction with glial cells. Therefore, the aim of the study is to evaluate the possible interaction between BoNT-A and glial cells through the mark of expression of cleaved-SNAP-25, P2X7, and TRPV1 by immunofluorescence, in rats induced to persistent hypernociception. To achieve this goal, Wistar rats will be induced to persistent hypernociception in the Temporomandibular joint and then, treated with an intra-articular injection of BoNT-A (7U / kg). Behavioral tests will be applied using the rat grimace scale and animals will be euthanized to collect samples of the trigeminal ganglion and subnucleus caudalis to mark the expression of the cleaved-SNAP-25, P2X7, and TRPV1 by immunofluorescence. Data will be collected and the corresponding statistical analyzes will be applied.
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