Advanced search
Start date

Transcriptional YgiV role and VisP protein-protein interaction on chemical signaling and LPS assembly in Salmonella enterica sorovar Typhimurium pathogenesis

Grant number: 16/12744-2
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): August 01, 2018
Effective date (End): October 31, 2020
Field of knowledge:Health Sciences - Pharmacy
Principal Investigator:Cristiano Gallina Moreira
Grantee:Patrick da Silva
Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
Associated research grant:14/06779-2 - Role of chemical signaling and auxiliary mechanisms in Salmonella enterica serovar Typhimurium virulence and other enteropathogens, AP.JP
Associated scholarship(s):18/22799-4 - Characterization and strength prediction of promoters regulated by YgiV transcriptional factor of Salmonella enterica serovar Typhimurium, BE.EP.DR


Chemical signaling is a mechanism employed by several bacterial species to interact within surrounding microbiota and their host. Upon this interaction the pathogenic bacteria regulate their virulence traits. The two-component system QseBC was described on chemical signaling in Salmonella enterica serovar Typhimurium, and a novel branch of pathogenic cascade regulation was revealed. Among these mechanisms a novel protein was described, VisP (Virulence and stress related Periplasmic protein). Previously, it was demonstrated the role of VisP on stress response and its relevance on pathogenicity mediated by SPI-1 and SPI-2 (Salmonella Patogenicity Island I and II). VisP is encoded by a gene inside a operon, which also presents ygiV, translated in a AraC-family transcriptional regulator. The O-antigen of the LPS layer provides protection against host defenses, and particularly its chain's length plays an essential role. O-antigen assembly is mediated by a group of periplasmic proteins, which mechanism of action was not completely elucidated. Preliminary data shows elevated levels of long and very-long O-antigen (L-Oag and VL-Oag) chains on ”visP mutant. The present study aims to better understand VisP role on O-antigen chains assembly and modality, as also presets the transcriptional regulation mediated by YgiV, correlating with pathogenicity. The full comprehension of this mechanisms of bacterial membrane homeostasis and the complex relationship amongst pathogenic bacteria, the microbiota, and the host is an essential topic in microbial pathogenesis. The complete elucidation of these signals will be essential to understand their relationship, develop novel technologies and therapies.

News published in Agência FAPESP Newsletter about the scholarship:
Articles published in other media outlets (0 total):
More itemsLess items

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SERAFIM, RODOLFO BORTOLOZO; DA SILVA, PATRICK; CARDOSO, CIBELE; DI CRISTOFARO, LUIS FERNANDO MACEDO; NETTO, RENATO PETITTO; DE ALMEIDA, RODRIGO; NAVEGANTE, GEOVANA; STORTI, CAMILA BALDIN; DE SOUSA, JULIANA FERREIRA; DE SOUZA, FELIPE CANTO; et al. Expression Profiling of Glioblastoma Cell Lines Reveals Novel Extracellular Matrix-Receptor Genes Correlated With the Responsiveness of Glioma Patients to Ionizing Radiation. FRONTIERS IN ONCOLOGY, v. 11, . (13/08135-2, 18/05018-9, 13/13465-1, 19/24335-8, 18/22799-4, 16/12744-2, 17/15208-7)
SERIBELLI, AMANDA APARECIDA; MACHADO RIBEIRO, TAMARA R.; DA SILVA, PATRICK; MARTINS, ISABELA MANCINI; VILELA, FELIPE PINHEIRO; CAZENTINI MEDEIROS, I, MARTA; PERONNI, KAMILA CHAGAS; DA SILVA JUNIOR, WILSON ARAUJO; MOREIRA, CRISTIANO GALLINA; FALCAO, JULIANA PFRIMER. Salmonella Typhimurium ST313 isolated in Brazil revealed to be more invasive and inflammatory in murine colon compared to ST19 strains. JOURNAL OF MICROBIOLOGY, v. 59, n. 9, . (17/06633-6, 16/12744-2, 19/19338-8, 19/03049-7, 16/24716-3)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SILVA, Patrick da. Transcriptional YgiV role and VisP protein-protein interaction on chemical signaling and LPS assembly in Salmonella enterica sorovar Typhimurium pathogenesis. 2020. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Farmacêuticas. Araraquara Araraquara.

Please report errors in scientific publications list by writing to: