Comparison of the effect of photobiomodulation and diclofenac treatment on the inflammatory process in an experimental model of traumatic muscular injury in diabetic rats

Abstract

Diabetes mellitus is a heterogeneous group of metabolic disorders that present hyperglycemia, characterized by inadequate insulin secretory response, defective insulin action, or both. Such a situation may result in delays in the tissue repair process after musculoskeletal injury, altering its morphology and resulting in an exacerbated inflammatory process. Different types of pharmacological and non-pharmacological therapies have been used in the treatment of muscular injuries, such as anti-inflammatory drugs and photobiomodulation therapy. However, we have little knowledge about the inflammatory process after an injury in diabetic organism. The objective of this project is to analyze the action of photobiomodulation therapy alone or in combination with diclofenac in the inflammatory process, due to muscle injury by trauma in rats induced to diabetes. Induction of diabetes will be by administration of streptozotocin (50 mg/kg). The rats will be injured in the right pelvic limb. The application of the photobiomodulation therapy will be performed in the trauma region, one hour after the induction of muscle injury (3J), for 30s. Diclofenac will be applied one hour after the lesion protocol (11.6 mg/g -1) topically. Gene expressions of TNF-±, IL-1², IL-6, IL-10 and myostatin will be analyzed, which will be quantified by real-time polymerase chain reaction (PCR). All analyzes will be performed at times of 24, 48 and 96 hours after the injury induction. (AU)