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Identification and quantification of antimicrobial resistance genes in the microbiome of seabirds from the Southern-Southeastern coast of Brazil

Grant number: 16/20956-0
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): June 01, 2018
Effective date (End): June 30, 2020
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Pathology
Principal researcher:Jose Luiz Catao Dias
Grantee:Ana Carolina Ewbank
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated scholarship(s):18/25885-9 - Identification and quantification of antimicrobial resistance genes in the microbiome of wild seabirds from Brazil: how do anthropogenic impacts affect these parameters?, BE.EP.DR

Abstract

The emergence of antibiotic resistance is considered a threat to human and animal health. Through horizontal trasfer, environmental antibiotic contamination may promote the exchange of genes between non-pathogenic and pathogenic environmental bacteria. The resulting remodeling of existing microbiomas leads to evolutionary and environmental complications that in association with their dissemination abilities classify genes of antibiotic resistance (ARGs) as environmental contaminants. Seabirds rapidly respond to environmental changes, being excellent marine ecossystem health biomarkers. Wildlife rehabilitation centers have a controverse role on the possible dissemination of bacteria presenting pathogenic genes and ARGs, both in the internal environment and external environment, after these animals are released. We intend on evaluating the degree of human interference in the marine ecossystem through the presence, identification and subsequent quantification of seleced ARGs (tetA, tetB, tetY, tetK, tetM, tetQ, sulI, sulII, str, aadA, catI, catII, erm(B), erm(F), qnrB, qnrS, blaCTX, blaTEM, mecA and mcr-1) present in plasmids of the microbiome of free ranging wild seabirds of two distinct groups: admitted in rehabilitation centers of the southern brazilian coast and residents of seabird colonies in the southeastern coast. For this purpose, we will employ real-time PCR (rtPCR) on fresh fecal samples (enema) collected (I) upon the individual's admission to the rehabilitation center; (II) 24 hours after initiation of the antimicrobial treatment; (III) 24 hrs after antimicrobial treatment termination and (IV) upon release, and of E. coli colonies previously isolated from several seabird species and stored in the Laboratory of Wildlife Comparative Pathology - LAPCOM/FMVZ/USP. Our goal is to determine the degree of environmental contamination by the ARGs present in seabirds at their habitat/admission, as well as the influence of antimicrobial treatment and/or stay in rehabilitation centers in regards to the presence and quantity of ARGs. (AU)

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Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
NOVOSELECKI, HELENA EXPOSTO; CATAO-DIAS, JOSE LUIZ; EWBANK, ANA CAROLINA; NAVAS-SUAREZ, PEDRO ENRIQUE; DUARTE-BENVENUTO, ARICIA; LIAL, HENRIQUE CHRISTINO; SILVA, SAMIRA COSTA; SANCHEZ-SARMIENTO, ANGELICA MARIA; GRAVENA, WALESKA; FERREIRA DA SILVA, VERA MARIA; CARVALHO, VITOR L.; MARMONTEL, MIRIAM; BERTOZZI, CAROLINA P.; RIBEIRO, VANESSA LANES; DO VALLE, RODRIGO DEL RIO; MARIGO, JULIANA; DAS NEVES, CARLOS G.; ESPERON, FERNANDO; SACRISTAN, CARLOS. ighly divergent herpesviruses in threatened river dolphins from Brazi. SCIENTIFIC REPORTS, v. 11, n. 1 DEC 31 2021. Web of Science Citations: 0.
SACRISTAN, CARLOS; COSTA-SILVA, SAMIRA; REISFELD, LAURA; NAVAS-SUAREZ, PEDRO ENRIQUE; EWBANK, ANA CAROLINA; DUARTE-BENVENUTO, ARICIA; COELHO COUTO DE AZEVEDO FERNANDES, NATALIA; ALBERGARIA RESSIO, RODRIGO; ANTONELLI, MARZIA; ROCHA LORENCO, JANAINA; FAVERO, CINTIA MARIA; MARIGO, JULIANA; KOLESNIKOVAS, CRISTIANE KIYOMI MIYAJI; CATAO-DIAS, JOSE LUIZ. Novel alphaherpesvirus in a wild South American sea lion (Otaria byronia) with pulmonary tuberculosis. Brazilian Journal of Microbiology, v. 52, n. 4 SEP 2021. Web of Science Citations: 0.
EWBANK, ANA CAROLINA; ESPERON, FERNANDO; SACRISTAN, CARLOS; SACRISTAN, IRENE; NEVES, ELENA; COSTA-SILVA, SAMIRA; ANTONELLI, MARZIA; LORENCO, JANAINA ROCHA; KOLESNIKOVAS, CRISTIANE K. M.; CATAO-DIAS, JOSE LUIZ. Occurrence and Quantification of Antimicrobial Resistance Genes in the Gastrointestinal Microbiome of Two Wild Seabird Species With Contrasting Behaviors. FRONTIERS IN VETERINARY SCIENCE, v. 8, MAR 22 2021. Web of Science Citations: 0.
EWBANK, ANA CAROLINA; ESPERON, FERNANDO; SACRISTAN, CARLOS; SACRISTAN, IRENE; KRUL, RICARDO; DE MACEDO, EDUARDO CAVALCANTE; CALATAYUD, OLGA; BUENO, IRENE; STREFEZZI, RICARDO DE FRANCISCO; CATAO-DIAS, JOSE LUIZ. Seabirds as anthropization indicators in two different tropical biotopes: A One Health approach to the issue of antimicrobial resistance genes pollution in oceanic islands. Science of The Total Environment, v. 754, FEB 1 2021. Web of Science Citations: 0.
EWBANK, ANA CAROLINA; SACRISTAN, CARLOS; COSTA-SILVA, SAMIRA; ANTONELLI, MARZIA; LORENCO, JANAINA R.; NOGUEIRA, GUILHERME A.; EBERT, MARIANA B.; KOLESNIKOVAS, CRISTIANE K. M.; CATAO-DIAS, JOSE LUIZ. Postmortem findings in Magellanic penguins (Spheniscus magellanicus) caught in a drift gillnet. BMC Veterinary Research, v. 16, n. 1 MAY 24 2020. Web of Science Citations: 0.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
EWBANK, Ana Carolina. Identification and characterization of antimicrobial resistant genes in the microbiome of seabirds of the Brazilian coast. 2021. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) São Paulo.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.