Protein gene expression by stem cells from pulp of deciduous teeth after the use of low intensity laser

Abstract

This study aimed to evaluate the effect of different energy densities of Low Intensity Laser on cell viability and proliferation of stem cell from human exfoliated deciduous teeth and dental pulp cells; on the RNAm expression of FGF2, VEGF, DSPP and DMP-1; and on the protein expression of FGF2, VEGF, DSPP and DMP-1. Cells samples will be obtained and grown in MEM± e DMEM culture medium with Fetal Bovine Serum (FBS). After the fourth passage, the sample will be irradiated with low intensity laser to the following groups: Group 1(1.2 J/cm2 - 05 mW - 10s), Group 2(2.5 J / cm2 - 05 mW - 20s), Group 3(3.7 J/cm2 - 05 mW - 30s), Group 4(5.0 J/cm2 - 05 mW - 40s), Group 5(6.2 J/cm2 - 05 mW - 50s), Group 6(2.5 J/cm2 - 10 mW - 10s), Group 7(3.7 J/cm2 - 15 mW - 10s), Group 8( 5.0 J/cm2 - 20 mW - 10 sec), Group 9 (6.2 J/cm2 - 25 mW - 10 sec), Positive Control (MEM± +10% FBS - no irradiated), Negative Control (MEM± +1% FBS - no irradiated). The techniques used for the analysis will be MTT, Crystal Violet, RT-PCR, ELISA, Western Blot and Immunofluorescence. The values in triplicate will be analyzed by ANOVA followed by Tukey test and Kruskal-Wallis analysis complemented by Dunn's test when obey normal and non-normal distribution, respectively. The significance level of 5% will be adopted for the differences considered statistically significant. (AU)