Advanced search
Start date
Betweenand

Host adaption of Mycobacterium tuberculosis and Mycobacterium bovis: a genomic and transcriptional approach

Grant number: 17/04617-3
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): May 01, 2018
Effective date (End): April 30, 2022
Field of knowledge:Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
Principal Investigator:Ana Marcia de Sá Guimarães
Grantee:Cristina Kraemer Zimpel
Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:16/26108-0 - Systems and comparative biology of Mycobacterium tuberculosis complex: effects of genetic variability on bacterial phenotype, AP.JP
Associated scholarship(s):19/10896-8 - Transcriptomic and metabolic features of Mycobacterium tuberculosis and Mycobacterium bovis during human macrophage infection, BE.EP.DR

Abstract

Tuberculosis is caused by members of the Mycobacterium Tuberculosis Complex (MTBC), a bacterial group that comprises twelve pathogen species that infect human beings and/or animals. The most frequent causative species of the disease are M. tuberculosis and M. bovis. Members of this complex evolved clonally from a M. tuberculosis ancestor and have high genomic identity, differing only by Single Nucleotide Polymorphisms (SNPs) and regions of difference (indels, RDs). Despite this high genetic similarity, MTBC members demonstrate phenotypic variations related to virulence and host tropism. While M. tuberculosis is highly adapted to human beings (i.e. a specialist bacterium) and is the major causative agent of the disease in this population, M. bovis shows unrestricted host predilection (i.e. a generalist bacterium), and can affect several animal species, including human beings, but with variable populational persistence. Although several reservoir animal species have been described for M. bovis, this pathogen does not persist in the human population; M. bovis transmission among patients is considered rare, even in cases of pulmonary disease. Therefore, in order to explain the unrestricted host preference of M. bovis and why this pathogen does not persist in the human population, this project aims (i) to sequence and annotate the genomes of M. bovis isolated from different non-bovine species and to compare to M. bovis genomes isolated from bovines and strains of M. tuberculosis, and (ii) to compare the global transcriptional profiles of M. bovis and M. tuberculosis during human macrophage infections and identify their activated metabolic pathways using Flux Balance Analysis (FBA). The comparative analysis to be performed herein will help elucidate the host-pathogen interaction of M. bovis and M. tuberculosis, and to identify genes related to macrophage infection that may serve as targets for treatment protocols and vaccine development. (AU)

News published in Agência FAPESP Newsletter about the scholarship:
Articles published in other media outlets (0 total):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ZIMPEL, CRISTINA KRAEMER; PATANE, JOSE SALVATORE L.; PROENGA GUEDES, AURELIANO COELHO; DE SOUZA, ROBSON F.; SILVA-PEREIRA, TAIANA T.; SOLER CAMARGO, NAILA C.; DE SOUZA FILHO, ANTONIO F.; IKUTA, CASSIA Y.; FERREIRA NETO, JOSE SOARES; SETUBAL, JOAO CARLOS; et al. Global Distribution and Evolution of Mycobacterium bovis Lineages. FRONTIERS IN MICROBIOLOGY, v. 11, . (17/04617-3, 17/20147-7)
SILVA-PEREIRA, TAIANA T.; IKUTA, CASSIA Y.; ZIMPEL, CRISTINA K.; CAMARGO, NAILA C. S.; DE SOUZA FILHO, ANTONIO F.; FERREIRA NETO, JOSE S.; HEINEMANN, MARCOS B.; GUIMARAES, ANA M. S.. Genome sequencing of Mycobacterium pinnipedii strains: genetic characterization and evidence of superinfection in a South American sea lion (Otaria flavescens). BMC Genomics, v. 20, n. 1, . (17/04617-3, 17/20147-7)
LIMA, DAIANE A. R.; ZIMPEL, CRISTINA K.; PATANE, JOSE S.; SILVA-PEREIRA, TAIANA TAINA; ETGES, RODRIGO N.; RODRIGUES, RUDIELLE A.; DAVILA, ALBERTO M. R.; IKUTA, CASSIA Y.; NETO, JOSE S. FERREIRA; GUIMARAES, ANA MARCIA S.; et al. Genomic analysis of an outbreak of bovine tuberculosis in a man-made multi-host species system: A call for action on wildlife in Brazil. TRANSBOUNDARY AND EMERGING DISEASES, . (17/04617-3, 19/10896-8)
GUIMARAES, ANA M. S.; ZIMPEL, CRISTINA K.. Mycobacterium bovis: From Genotyping to Genome Sequencing. MICROORGANISMS, v. 8, n. 5, . (16/26108-0, 17/04617-3)
CARNEIRO, PAULO ALEX; ZIMPEL, CRISTINA KRAEMER; PASQUATTI, TAYNARA NUNES; SILVA-PEREIRA, TAIANA T.; TAKATANI, HARUO; SILVA, CHRISTIAN B. D. G.; ABRAMOVITCH, ROBERT B.; SA GUIMARAES, ANA MARCIA; DAVILA, ALBERTO M. R.; ARAUJO, FLABIO R.; et al. Genetic Diversity and Potential Paths of Transmission of Mycobacterium bovis in the Amazon: The Discovery of M. bovis Lineage Lb1 Circulating in South America. FRONTIERS IN VETERINARY SCIENCE, v. 8, . (16/26108-0, 19/10896-8, 17/04617-3)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
ZIMPEL, Cristina Kraemer. Host adaptation of Mycobacterium tuberculosis and Mycobacterium bovis: a genomic and transcriptional approach. 2022. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) São Paulo.

Please report errors in scientific publications list using this form.