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Changes in the metabolic profile of kidney in response to exercise after fluoride exposure in mice with different susceptibility to dental fluorosis

Grant number: 18/06168-4
Support Opportunities:Scholarships abroad - Research Internship - Post-doctor
Effective date (Start): June 01, 2018
Effective date (End): September 16, 2018
Field of knowledge:Health Sciences - Dentistry - Social and Preventive Dentistry
Principal Investigator:Marília Afonso Rabelo Buzalaf
Grantee:Mileni da Silva Fernandes
Supervisor: Jiri Adamec
Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
Research place: University of Nebraska-Lincoln (UNL), United States  
Associated to the scholarship:15/12109-2 - Effect of exercise on metabolism of fluoride in mice with different susceptibility to dental fluorosis: proteomic analysis., BP.PD


The strains of mice A/J and 129P3/J have been investigated because they respond quite differently to fluoride (F) exposure. F is an extremely important element in terms of public health due to its properties to prevent or reverse caries in individuals of all ages. It can offer risks to the body if consumed or applied indiscriminately or inappropriately, such as dental fluorosis. After its absorption, F is distributed through the blood stream and stored in calcified tissues and soft tissues, and its excretion is essentially via the kidney. Several molecular mechanisms involving the F metabolism are still unknown. In the last decade, many studies have sought to unravel the influence of genetic factors in genetic susceptibility to dental fluorosis using mouse strains that have very different responses to the effects F. Thus, there is evidence that resistance and susceptibility to fluorosis seem to be influenced by genetic factors as well as the interaction with the environment, such as physical activity, and diet. Metabolomics has shown rapid growth in its application to human health research because of fundamental advancements in measurement and analysis techniques. Metabolomics has unique and proven advantages in systems biology and biomarker discovery. In this study we propose through metabolomics analysis, identify metabolic waste products that are normally cleared by the kidney of mice susceptible or resistant to fluorosis in response to changes in exposure F, with and without exercise. Therefore, weanling male A/J mice (susceptible to dental fluorosis, n = 18) and 129P3/J mice (resistant, n = 18), were divided into six groups according to each treatment, received the drinking water containing 0 or 50 ppm F, for 42 days. The groups with physical activity were held daily races on a treadmill for 60 minutes. After the completion of the treatment, the animals were euthanized, for collecting samples and conducting analyzes.

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