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Genetic engineering of yeast for conversion lignocellulosic material to ferulic acid

Grant number: 17/08166-6
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): April 01, 2018
Effective date (End): April 30, 2022
Field of knowledge:Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
Principal Investigator:Fábio Márcio Squina
Grantee:Nathália Vilela
Host Institution: Pró-Reitoria Acadêmica. Universidade de Sorocaba (UNISO). Sorocaba , SP, Brazil
Associated research grant:15/50590-4 - Lignin valorization in cellulosic ethanol plants: biocatalytic conversion via ferulic acid to high value chemicals, AP.BIOEN.TEM
Associated scholarship(s):20/10593-2 - Application of CRISPR/Cas9-based tools to develop a Saccharomyces cerevisiae strain capable of producing 4-vinylguaiacol from lignocellulose hydrolysates, BE.EP.DR


The research for renewable sources of energy is constantly due to the fact that fossil fuels cause several problems that affect the environment, the economy and the society. In this scenario, lignocellulosic biomass takes some advantages, since this material consists of cellulose and hemicellulose that can be used for ethanol production, in addition to lignin, which can be converted into high chemical compounds. Despite of having a high recalcitrance owing its complex structure of polymers, lignin can be degraded by some oxidative enzymes of bacteria and fungi, such as peroxidases. Thus, this work aims the genetic improvement of yeast Rhodosporidium sp., a microorganism capable of degrading lignin, in order to develop a process of conversion of lignocellulosic material to ferulic acid. This compound is a precursor of chemical compounds used in the industry, such as L-DOPA (L-3,4-dihydroxyphenylalanine) and coniferyl alcohol. The evaluation of the enzymatic system and the lignin conversion pathways in this yeast will serve as the basis for silencing and overexpression of genes using the CRISPR/Cas9 system. (AU)

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SODRE, VICTORIA; VILELA, NATHALIA; TRAMONTINA, ROBSON; SQUINA, FABIO MARCIO. icroorganisms as bioabatement agents in biomass to bioproducts application. BIOMASS & BIOENERGY, v. 151, . (16/07926-4, 18/18101-1, 15/50590-4, 17/08166-6)
DOS SANTOS, OTAVIO AUGUSTO LEITAO; GONCALVES, THIAGO AUGUSTO; SODRE, VICTORIA; VILELA, NATHALIA; TOMAZETTO, GEIZECLER; SQUINA, FABIO M.; GARCIA, WANIUS. Recombinant expression, purification and characterization of an active bacterial feruloyl-CoA synthase with potential for application in vanillin production. Protein Expression and Purification, v. 197, p. 7-pg., . (17/16089-1, 15/50590-4, 18/18101-1, 17/08166-6, 20/15595-3, 20/05784-3, 17/17275-3)
LIBERATO, MARCELO VIZONA; PRATES, ERICA TEIXEIRA; GONCALVES, THIAGO AUGUSTO; BERNARDES, AMANDA; VILELA, NATHALIA; FATTORI, JULIANA; EMATSU, GABRIELA CRISTINA; CHINAGLIA, MARIANA; MACHI GOMES, EMERSON RODRIGO; MIGLIORINI FIGUEIRA, ANA CAROLINA; et al. Insights into the dual cleavage activity of the GH16 laminarinase enzyme class on beta-1,3 and beta-1,4 glycosidic bonds. Journal of Biological Chemistry, v. 296, . (17/08166-6, 14/04105-4, 15/50612-8, 17/16089-1, 17/22669-0, 13/15582-5, 13/08293-7)
SODRE, VICTORIA; ARAUJO, JUSCEMACIA NASCIMENTO; GONCALVES, THIAGO AUGUSTO; VILELA, NATHALIA; KIMUS BRAZ, ANTONIO SERGIO; FRANCO, TELMA TEIXEIRA; NETO, MARIO DE OLIVEIRA; DE LIMA DAMASIO, ANDRE RICARDO; GARCIA, WANIUS; SQUINA, FABIO MARCIO. An alkaline active feruloyl-CoA synthetase from soil metagenome as a potential key enzyme for lignin valorization strategies. PLoS One, v. 14, n. 2, . (17/05901-7, 17/22669-0, 17/16976-8, 15/50612-8, 17/16089-1, 15/50590-4, 17/08166-6, 18/18101-1, 17/17275-3)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
VILELA, Nathália. A multiomic-based analysis of Rhodosporidium fluviale LM-2 yeast for lignin biocatalytic valorization. 2022. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.

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