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Proteome changes and DNA damage caused by photoinduced oxidative stress in cells

Grant number: 16/11430-4
Support Opportunities:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): April 01, 2018
Effective date (End): December 31, 2021
Field of knowledge:Biological Sciences - Biochemistry - Metabolism and Bioenergetics
Principal Investigator:Paolo Di Mascio
Grantee:Hellen Paula Valerio
Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:13/07937-8 - Redoxome - Redox Processes in Biomedicine, AP.CEPID


Solar UV radiation is composed of 95% UVA and 5% UVB light. It is still a matter of debate whether or not UVA light plays a role in carcinogenesis and photoaging of exposed skin. UVA light may be absorbed by cellular chromophores, giving rise to photoexcited species, such as singlet oxygen. These species are known mediators of oxidative stress. In this work, we aim to understand how the UVA component of UV radiation is associated with Skin Cancer and photoaging. Proteomic studies applied to photochemistry and photobiology are scarce. Therefore, we will use a type II photosensitizer incorporated into DNA - thioguanine - to analyze proteome alterations caused in cells exposed to UVA light. Proteome analysis will include differences in protein expression, protein-protein and protein-DNA crosslinks and post-translational modifications. Besides, we will also focus on oxidative damages caused by singlet oxygen on DNA. To this end, we will use state-of-the-art technologies, such as a new generation Orbitrap Fusion Lumos mass spectrometer and a confocal fluorescence microscope, which will allows us to localize intracellular production of 1O2. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
VALERIO, HELLEN PAULA; RAVAGNANI, FELIPE GUSTAVO; CANDELA, ANGELA PAOLA YAYA; DA COSTA, BRUNA DIAS CARVALHO; RONSEIN, GRAZIELLA ELIZA; DI MASCIO, PAOLO. Spatial proteomics reveals subcellular reorganization in human keratinocytes exposed to UVA light. ISCIENCE, v. 25, n. 4, p. 27-pg., . (15/07768-7, 16/00696-3, 16/11430-4, 12/12663-1, 13/07937-8)
VALERIO, HELLEN PAULA; RAVAGNANI, FELIPE GUSTAVO; RONSEIN, GRAZIELLA ELIZA; DI MASCIO, PAOLO. A single dose of Ultraviolet-A induces proteome remodeling and senescence in primary human keratinocytes. SCIENTIFIC REPORTS, v. 11, n. 1, . (15/07768-7, 13/07937-8, 12/12663-1, 16/11430-4, 16/00696-3)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
VALERIO, Hellen Paula. Proteomic analysis of human keratinocytes under UVA-induced stress. 2021. Doctoral Thesis - Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) São Paulo.

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