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Proteomic characterization of the effect of Bothrops jararaca snake venom on normal vs cancer cell lines

Grant number: 17/06496-9
Support type:Scholarships in Brazil - Master
Effective date (Start): April 01, 2018
Effective date (End): September 30, 2019
Field of knowledge:Biological Sciences - Pharmacology - Biochemical and Molecular Pharmacology
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:Leo Kei Iwai
Grantee:Carolina Yukiko Kisaki
Home Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
Associated research grant:13/07467-1 - CeTICS - Center of Toxins, Immune-Response and Cell Signaling, AP.CEPID

Abstract

Venoms and secretions of animals such as snakes, frogs, scorpions and spiders have been studied for their therapeutic potential and they have been used for the development of drugs for the treatment of several types of diseases such as diabetes and cancer. Specific toxins have already been isolated from snake venom and are the active components of some drugs for hypertension. In addition, there are currently several papers in the literature describing the anticancer potential of isolated and / or modified components of snake venoms in phase 2 and 3 clinical trials.Although there are numerous approaches involving the biochemical and physiological characterization of snake venom action on cell lines or tissues, no work has yet been described to map the proteome of cells under treatment with snake venoms. Thus, in this work, the proteome of several tumor cell lines treated or not with Bothrops jararaca snake venom will be characterized by mass spectrometry. In addition, we will characterize the comparative and quantitative differential proteomes of these lineages versus normal cells treated or not with the venom.The result of this analysis may reveal the action of B. jararaca venom at the molecular level that may be specific among different tumor cell lines or specific to normal cells. The mapping of specific proteins and altered pathways with the venom treatment will allow the characterization and detection of proteins or pathways that may be potential targets for therapeutic intervention to diagnose and treat cancer.Although there are numerous approaches involving the biochemical and physiological characterization of snake venom action on cell lines or tissues, no work has yet been described to map the proteome of cells under treatment with snake venoms. Thus, in this work, the proteome of several tumor cell lines treated or not with Bothrops jararaca snake venom will be characterized by mass spectrometry. In addition, we will characterize the comparative and quantitative differential proteomes of these lineages versus normal cells treated or not with the venom.The result of this analysis may reveal the action of B. jararaca venom at the molecular level that may be specific among different tumor cell lines and specific to normal cells. The mapping of specific proteins and altered pathways with the venom treatment will allow the characterization and detection of proteins or pathways that may be potential targets for therapeutic intervention to diagnose and treat cancer. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MONTONI, FABIO; ANDREOTTI, DIANA ZUKAS; DOS SANTOS EICHLER, ROSANGELA APARECIDA; SANTOS, WELLINGTON DA SILVA; KISAKI, CAROLINA YUKIKO; SUEHIRO ARCOS, STEPHANIE SANTOS; LIMA, ISMAEL FEITOSA; MALTAURO SOARES, MAGNA APARECIDA; NISHIYAMA-JR, MILTON YUTAKA; NAVA-RODRIGUES, DANIEL; FERRO, EMER SUAVINHO; CARVALHO, VALDEMIR MELECHCO; IWAI, LEO KEI. The impact of rattlesnake venom on mice cerebellum proteomics points to synaptic inhibition and tissue damage. JOURNAL OF PROTEOMICS, v. 221, JUN 15 2020. Web of Science Citations: 0.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.