Effect of proanthocyanidin gel applied over acquired enamel pellicle on initial erosion

Abstract

Due to the high prevalence of dental erosion found in recent years, it is necessary to search for preventive therapies for this alteration. Proanthocyanidin is a substance currently studied because its properties, which involves remineralization, reduction of demineralization and dentin biomodification. Thus, it may be a new alternative to prevent erosion in enamel and / or improve natural protective factors, such as the acquired pellicle. Therefore, the aim of this study will be to evaluate the effect of Proanthocyanidin on the enamel acquired pellicle in the initial erosion. The acquired pellicle will be formed in situ by the use of intraoral device by two volunteers for 2 hours. The enamel blocks of each study group (n = 16) will be treated in vitro according to the groups: G1- 6.5% Proanthocyanidin Gel + acquired pellicle formed in situ; G2- only 6.5% Proanthocyanidin Gel; G3- only acquired pellicle formed in situ; G4- no treatment (no gel and no acquired pellicle). Gels will be applied for 1 minute. Then the enamel blocks will be immersed in citric acid to promote a short erosive challenge to generate an enamel softening. The response variable used will be the surface hardness (percentage of hardness loss). The data will be submitted to the Analysis of Variance followed by the Tukey test if they follow the normal distribution parameters and homogeneity of the variances. (AU)