Evaluation of wound healing of wistar rats treated with honey and propolis's bee (Apis mellifera L.) associated with low-level laser therapy

Abstract

Healing is a physiological process of recovering of normality of the anatomical structure of the damaged tissue. Several studies have been done with natural substances because of their minimal side effects. The aims of the present work is to evaluate and analyze the healing properties of honey and propolis´s bee (Apis mellifera L.) in natura associated or not to therapeutic laser in surgically induced skin wounds of Wistar rats. It will be used 126 Wistar rats, adult males. The rats will be submitted to surgical induction of cutaneous wounds, and randomly divided into seven groups of 18 animals: Group 1 - wounds treat with saline solution (0.9%); Group 2 - wounds treat with healing ointment Alantol®; Group 3 - wounds treat with low-level laser therapy; Group 4 - wounds treat with mixture of honey in natura and hydroalcoholic propolis solution (5%) (MP); Group 5 - wounds treat with MP and low-level laser therapy; Group 6 - wounds treat with low-level laser therapy and honey in natura; Group 7 - wounds treat with low-level laser therapy and hydroalcoholic propolis solution (5%). Six animals from each group will undergo excisional biopsies after euthanasia at three different moments: seventh day, 14th day and 21st day after the start the treatment. Macroscopic, histological and magnetic resonance evaluations of the wounds will be performed. Statistical analysis will be used for analysis of variance (ANOVA) for repeated samples, with contrasts by Tukey test and Kolmogorov-Smirnov test. Friedman test will be used with data that do not assemble the normality characteristics. For comparison between the four groups of the study, analysis of variance for independent samples (ANOVA One way) for normal data, and Kruskal-Wallis test, for data outside the normality conditions will be used. Differences will be considered significant with p  0.05. (AU)