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Probiotics effect on the periodical lesion development induced in rats

Grant number: 17/08314-5
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): January 01, 2018
Effective date (End): May 07, 2019
Field of knowledge:Health Sciences - Dentistry - Endodontics
Principal researcher:João Eduardo Gomes Filho
Grantee:Leopoldo Cosme Silva
Home Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Probiotics are viable bacteria with proven benefits to overall health. During the last decade, the use of probiotics is also aimed at oral health. Studies report the benefits of these bacteria in the control of periodontal diseases and cavities. However, there is no study linking probiotics with endodontics and more specifically, the action of the probiotic on the periapical lesion. The objective of this study will be to analyze and compare the periapical lesion development in rats with normal diet or diet supplemented with probiotics. Twenty-four Wistar albino rats, all receiving the periapical lesion induction, will be allocated in 3 groups as follows: G1 (n = 8) normal diet; G2 (n = 8) systemic diet with probiotic (Lactobacillus rhamnosus) via gavage; G3 (n = 8) systemic diet with probiotic (Bifidobacterium longum) via gavage. Throughout the experimental period, the animals and the feed consumed will be weighed daily. After 30 days of injury induction and administration of the diets, collection of saliva, root canal and blood contents will be performed, after which the euthanasia of the animals will be analyzed in order to analyze and compare micrographically and histometrically the periapical lesion; analyze and compare the tissues of the periapical region of rat teeth using the expression of the TRAP, OPG and RANKL proteins as cellular indicators of predisposition to reabsorption; analyze and compare the tissues of the periapical region of rat teeth using the expression of cytokines and IL-1² and IL-10 as indicators of local inflammation; analyze and compare the expression of IL-1² and IL-10 cytokines as indicators of systemic inflammation; analyze and compare the neutrophil, eosinophil, lymphocyte and monocyte concentrations between the experimental groups by hematological analysis; and to analyze and compare the concentrations of the cellular activity markers of bone metabolism: calcium, phosphorus and alkaline phosphatase and analyze the microbiota of saliva and root canals.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LEOPOLDO COSME-SILVA; RENAN DAL-FABBRO; LUCIANO TAVARES ANGELO CINTRA; EDILSON ERVOLINO; ALANA SANT’ANA DO PRADO; DANIEL PINTO DE OLIVEIRA; PRISCYLLA GONÇALVES CORREIA LEITE DE MARCELOS; JOÃO EDUARDO GOMES-FILHO. Dietary supplementation with multi-strain formula of probiotics modulates inflammatory and immunological markers in apical periodontitis. Journal of Applied Oral Science, v. 29, p. -, 2021. Web of Science Citations: 0.
COSME-SILVA, L.; DAL-FABBRO, R.; CINTRA, L. T. A.; ERVOLINO, E.; PLAZZA, F.; MOGAMI BOMFIM, S.; DUARTE, P. C. T.; JUNIOR, V. E. D. S.; GOMES-FILHO, J. E. Reduced bone resorption and inflammation in apical periodontitis evoked by dietary supplementation with probiotics in rats. International Endodontic Journal, v. 53, n. 8 MAY 2020. Web of Science Citations: 0.
COSME-SILVA, L.; DAL-FABBRO, R.; CINTRA, L. T. A.; DOS SANTOS, V. R.; DUQUE, C.; ERVOLINO, E.; MOGAMI BOMFIM, S.; GOMES-FILHO, J. E. Systemic administration of probiotics reduces the severity of apical periodontitis. International Endodontic Journal, v. 52, n. 12 AUG 2019. Web of Science Citations: 0.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SILVA, Leopoldo Cosme. Effect of probiotics on the development of induced periapical lesion in rats. 2019. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba Araçatuba.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.