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Optimization of cello-oligosaccharides production by enzymatic hydrolysis of sugarcane straw via heterologous expression of cellulolytic and oxidative enzymes

Grant number: 17/16159-0
Support type:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): January 01, 2018
Effective date (End): October 31, 2020
Field of knowledge:Agronomical Sciences - Food Science and Technology - Food Engineering
Principal researcher:Rosana Goldbeck
Grantee:Fernando César Barbosa
Home Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated research grant:15/50612-8 - An integrated approach to explore a novel paradigm for biofuel production from lignocellulosic feedstocks, AP.TEM
Associated scholarship(s):19/02214-4 - Engineering and optimization of expression systems for intrinsically disordered proteins, BE.EP.DD


Bioethanol has been considered the main alternative to the crescent world demand for fuel because it is capable of reducing the environment impacts caused by non-renewable fuels, such as increased pollution and global warming. Based on that, the sugarcane straw represent a potential source of lignocellulosic biomass for 2nd generation bioethanol production, since it can represent more than 25 % of all the ethanol produced in Brazil per year. In addition, the enzymatic conversion of the lignocellulosic biomass in cello-oligosaccharides instead of saccharides is a field still under-researched. It may be an interesting alternative to the traditional transformation of biomass in glucose given its advantages, such as lower process contaminants, shorter total fermentation time, higher binding affinity to cellulosic substrates, and limited process inhibition by high glucose concentration. With this premise, this project has the main goal of optimize the production of cello-oligosaccharides by enzymatic hydrolysis of pretreated sugarcane straw via heterologous expression of cellulolytic and oxidative enzymes. For this, this project also aims to identify the key enzymes (cellulolytic and oxidative) required for degradation of pretreated straw (cellolignin) in cello-oligosaccharides; select interesting genes, clone and express in E.coli, as well as purify and test activity/function of novel enzyme candidates having key activities. Moreover, it will be performed the optimization of cello-oligosaccharides production by experimental designs of key enzymes mixtures. Finally, we expect to obtain a hydrolyzed rich in cello-oligosaccharides for further fermentation by Geobaccillus. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BARBOSA, FERNANDO CESAR; KENDRICK, EMANUELE; BRENELLI, LIVIA BEATRIZ; ARRUDA, HENRIQUE SILVANO; PASTORE, GLAUCIA MARIA; RABELO, SARITA CANDIDA; DAMASIO, ANDRE; FRANCO, TELMA TEIXEIRA; LEAK, DAVID; GOLDBECK, ROSANA. Optimization of cello-oligosaccharides production by enzymatic hydrolysis of hydrothermally pretreated sugarcane straw using cellulolytic and oxidative enzymes. BIOMASS & BIOENERGY, v. 141, . (17/22669-0, 15/50612-8, 17/16159-0, 19/08542-3)
BARBOSA, FERNANDO CESAR; SILVELLO, MARIA AUGUSTA; GOLDBECK, ROSANA. Cellulase and oxidative enzymes: new approaches, challenges and perspectives on cellulose degradation for bioethanol production. Biotechnology Letters, v. 42, n. 6, p. 875-884, . (18/20787-9, 19/08542-3, 17/16159-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
BARBOSA, Fernando César. Otimização da produção de celo-oligossacarídeos por hidrólise enzimática de palha de cana-de-açúcar pré-tratada utilizando enzimas celulolíticas e oxidativas. 2020. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia de Alimentos Campinas, SP.

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