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Effect of 4% titanium tetrafluoride varnish on the prevention of dentin demineralization under a cariogenic microcosm biofilm model - Micro-CT and TMR analysis

Grant number: 17/13386-5
Support type:Scholarships abroad - Research Internship - Master's degree
Effective date (Start): January 08, 2018
Effective date (End): February 23, 2018
Field of knowledge:Health Sciences - Dentistry - Social and Preventive Dentistry
Principal researcher:Ana Carolina Magalhães
Grantee:Daiana Moreli Soares dos Santos
Supervisor abroad: Annette Wiegand
Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
Research place: Göttingen University, Germany  
Associated to the scholarship:16/11152-4 - Evaluation of the antimicrobial effect of 4% titanium tetrafluoride varnish in a cariogenic microcosm biofilm model on dentine, BP.MS


The present study aims 1) to evaluate the effect of 4% TiF4 varnish compared to 5.42% NaF and 2% chlorexidine on the prevention of dentin demineralization under a cariogenic microcosm biofilm and 2) to validate the use of Micro-CT to measure dentin demineralization. For the microcosm biofilm formation, saliva will be collected from 2 healthy subjects, who must have not brush their teeth for the last 24h and not drink or eat for the last 2h. The saliva will be diluted (70% saliva and 30% glycerol), and aliquots of 1 ml will be stored at -80ºC. Bovine root dentine samples (4 mm x 4 mm) will be prepared and treated for 6h with the varnishes containing: A) 4% TiF4 (pH 1.0, 2.45% F); B) 5.42% NaF (pH 5.0, 2.45% F); C) placebo D) 2% chlorexidine gel - positive control or E) untreated - negative control (n=16). The dentin samples will be exposed to human saliva mixed with McBain saliva (1:50) containing 0.2% sucrose (v=1.5 ml) for the microcosm biofilm formation, using 24-wells plates for period that will be defined (pilot study with 5, 10 and 15 days). The dentin demineralization will be measured using Micro-CT, at the University of Göttingen, GER and using transverse microradiography-TMR at the University of São Paulo, BR. The data will be plotted and submitted to the statistical analysis (p<0.05).

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SOARES DOS SANTOS, DAIANA M.; BRAGA, ALINE S.; RIZK, MARTA; WIEGAND, ANNETTE; MAGALHAES, ANA C.. Comparison between micro-computed tomography and transverse microradiography of sound dentine treated with fluorides and demineralized by microcosm biofilm. European Journal of Oral Sciences, . (17/13386-5, 17/00556-0)

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