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Evaluating chronic administration of melanin concentrating hormone (MCH) effects in lactating Long-Evans rats

Grant number: 17/17998-5
Support Opportunities:Scholarships in Brazil - Post-Doctorate
Effective date (Start): October 01, 2017
Effective date (End): July 05, 2022
Field of knowledge:Biological Sciences - Physiology - Physiology of Organs and Systems
Principal Investigator:Jackson Cioni Bittencourt
Grantee:Marianne Orlandini Klein
Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:16/02224-1 - The existence of neurogenesis during the lactation period, AP.TEM


The melanin-concentrating hormone (MCH) is a peptide known to act, among other functions, in regulating the energy balance. Only during lactation, this neuropeptide is synthesized in the ventromedial preoptic area (MPOAvm), a region directly linked to the expression of maternal behavior. However, the role that MCH exerts during lactation, a period of high energy demand, is not elucidated. It is believed that it may be related to signaling termination of lactation for the mother. For this, Long-Evans rats will receive, through osmotic minipumps, chronic administrations of MCH, NEI, the combination of both (MCH+NEI) and the MCH antagonist, by intracerebroventricular injections. The animals will have their behavior recorded to evaluate the appetitive and consummatory aspects of maternal care. The dietary intake of females will be monitored throughout the period. On specific days, milk will be collected for volumetric and nutritional analyzes. It is believed that MCH may also be involved in the mechanism of milk ejection. The females 'brains will be collected for in situ hybridization, immunohistochemistry (MCH-ir), western blotting and qRT-PCR techniques to identify the peptide synthesis and its precursor in specific areas of the females' brains. Combined with behavioral assessments, this study will allow us to have a more detailed and temporal view of the action of MCH during the physiological process of lactation.

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Scientific publications (9)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BATTAGELLO, DANIELLA S.; LORENZON, ALINE R.; DINIZ, GIOVANNE B.; MOTTA-TEIXEIRA, LIVIA C.; KLEIN, MARIANNE O.; FERREIRA, JOZELIA G. P.; ARIAS, CARLOS M.; ADAMANTIDIS, ANTOINE; SITA, V, LUCIANE; CIPOLLA-NETO, JOSE; et al. The Rat Mammary Gland as a Novel Site of Expression of Melanin-Concentrating Hormone Receptor 1 mRNA and Its Protein Immunoreactivity. FRONTIERS IN ENDOCRINOLOGY, v. 11, . (15/05990-4, 16/02748-0, 17/16293-8, 10/52068-0, 16/04210-8, 17/17998-5, 14/50457-0, 12/04554-8, 14/13052-1, 16/18941-4, 16/19845-9, 16/02224-1)
DINIZ, GIOVANNE B.; CANDIDO, PAULO L.; KLEIN, MARIANNE O.; ALVISI, RENATO D.; PRESSE, FRANCOISE; NAHON, JEAN-LOUIS; FELICIO, LUCIANO F.; BITTENCOURT, JACKSON C.. The weaning period promotes alterations in the orexin neuronal population of rats in a suckling-dependent manner. Brain Structure & Function, v. 223, n. 8, p. 3739-3755, . (17/17998-5, 16/02224-1, 16/02748-0, 10/52068-0)
KLEIN, MARIANNE O.; BATTAGELLO, DANIELLA S.; CARDOSO, ARIEL R.; HAUSER, DAVID N.; BITTENCOURT, JACKSON C.; CORREA, RICARDO G.. Dopamine: Functions, Signaling, and Association with Neurological Diseases. Cellular and Molecular Neurobiology, v. 39, n. 1, p. 31-59, . (16/02224-1, 17/18019-0, 17/17998-5)
WASINSKI, FREDERICK; KLEIN, MARIANNE O.; BITTENCOURT, JACKSON C.; METZGER, MARTIN; DONATO JR, JOSE. Distribution of growth hormone-responsive cells in the brain of rats and mice. Brain Research, v. 1751, . (16/20897-3, 16/02224-1, 17/16473-6, 17/17998-5, 17/02983-2)
CAMPOS, ANA M. P.; TEIXEIRA, PRYSCILA D. S.; WASINSKI, FREDERICK; KLEIN, MARIANNE O.; BITTENCOURT, JACKSON C.; METZGER, MARTIN; DONATO, JR., JOSE. Differences between rats and mice in the leptin action on the paraventricular nucleus of the hypothalamus: Implications for the regulation of the hypothalamic-pituitary-thyroid axis. Journal of Neuroendocrinology, . (16/20897-3, 16/02224-1, 17/16473-6, 17/02983-2, 17/17998-5)
BATTAGELLO, DANIELLA S.; DRAGUNAS, GUILHERME; KLEIN, MARIANNE O.; AYUB, ANA L. P.; VELLOSO, FERNANDO J.; CORREA, RICARDO G.. Unpuzzling COVID-19: tissue-related signaling pathways associated with SARS-CoV-2 infection and transmission. Clinical Science, v. 134, n. 16, p. 2137-2160, . (17/17998-5, 17/16549-2, 19/05641-0)
CAMPOS, ANA M. P.; WASINSKI, FREDERICK; KLEIN, MARIANNE O.; BITTENCOURT, JACKSON C.; METZGER, MARTIN; DONATO JR, JOSE. Fasting reduces the number of TRH immunoreactive neurons in the hypothalamic paraventricular nucleus of male rats, but not in mice. Neuroscience Letters, v. 752, . (16/02224-1, 17/16473-6, 17/17998-5, 16/20897-3)
DINIZ, GIOVANNE B.; BATTAGELLO, DANIELLA S.; KLEIN, MARIANNE O.; BONO, BIANCA S. M.; FERREIRA, JOZELIA G. P.; MOTTA-TEIXEIRA, LIVIA C.; DUARTE, JESSICA C. G.; PRESSE, FRANCOISE; NAHON, JEAN-LOUIS; ADAMANTIDIS, ANTOINE; et al. Ciliary melanin-concentrating hormone receptor 1 (MCHR1) is widely distributed in the murine CNS in a sex-independent manner. Journal of Neuroscience Research, v. 98, n. 10, . (15/05990-4, 16/02748-0, 17/16293-8, 17/17998-5, 16/18941-4, 16/19845-9, 16/02224-1, 14/22313-3)
DINIZ, GIOVANNE B.; BATTAGELLO, DANIELLA S.; CHERUBINI, PEDRO M.; REYES-MENDOZA, JULIO D.; LUNA-ILLADES, CESAR; KLEIN, MARIANNE O.; MOTTA-TEIXEIRA, LIVIA C.; SITA, LUCIANE V.; MIRANDA-ANAYA, MANUEL; MORALES, TERESA; et al. Melanin-concentrating hormone peptidergic system: Comparative morphology between muroid species. JOURNAL OF COMPARATIVE NEUROLOGY, v. 527, n. 18, p. 2973-3001, . (16/02224-1, 16/02748-0, 14/22313-3, 17/17998-5, 16/18941-4, 17/16293-8)

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