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Evaluation of multidrug resistance of bacteria isolated from hospital and domestic effluent: determination of survival to the treatment of sewage and inactivating antibiotic enzymes

Grant number: 17/10723-0
Support type:Scholarships in Brazil - Master
Effective date (Start): November 01, 2017
Effective date (End): January 31, 2019
Field of knowledge:Health Sciences - Nursing - Public Health Nursing
Principal researcher:Susana Segura Muñoz
Grantee:Guilherme Sgobbi Zagui
Home Institution: Escola de Enfermagem de Ribeirão Preto (EERP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Antibiotics may be present in the sewage as they are excreted by the body fluids of users of these drugs and characterize this environment as selective for multiresistant bacteria. The sewage treatment plants aim at the removal of chemical contaminants and microorganisms, however they can represent a gene exchange reactor and favor resistance to antibiotics and sewage treatment used. Spectrum-extended ²-lactamase (ESBL) and Klebsiella pneumoniae carbapenemase (KPC) comprise a group of enzymes that degrade potent antibiotics which represents a serious public health problem worldwide. In this context, the present study aims at the identification of Gram-negative multiresistant bacteria in the hospital and domestic sewage, as well as the survival of the bacteria to the treatment of sewage and the phenotypic determination of ESBL and KPC. The identification process will provide for the isolation of bacteria in MacConkey Agar, Salmonella-Shigella, Cetremide and TCBS media, and biochemical tests of Bactray kits (Laborclin®). From the identification will be performed the antibiotic with antibiotics ampicillin, amoxicillin, cefotaxime, cefoxetine, ceftazidime, ceftazidime, ciprofloxacin, chloramphenicol, ertapenem, gentamicin, imipenem, meropenem, rifampicin, sulfazotrim and tetracycline. The phenotypic identification of ESBL and KPC will be, respectively, by the disc diffusion method and modified Hodge test. It is expected to find bacteria of the family Enterobacteriaceae, with higher prevalence of Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa resistant to ²-lactam and carbapenems. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
G. S. ZAGUI; K. A. A. TONANI; B. M. FREGONESI; G. P. MACHADO; T. V. SILVA; L. N. ANDRADE; D. ANDRADE; S. I. SEGURA-MUÑOZ. Esgoto de hospital terciário como reservatório de bactérias expressando o fenótipo MDR no Brasil. Brazilian Journal of Biology, v. 82, . (17/10723-0)
ZAGUI, GUILHERME SGOBBI; DE ANDRADE, LEONARDO NEVES; MOREIRA, NATALIA COLUMBARO; SILVA, THAIS VILELA; MACHADO, GABRIEL PINHEIRO; DA COSTA DARINI, ANA LUCIA; SEGURA-MUNOZ, SUSANA INES. Gram-negative bacteria carrying beta-lactamase encoding genes in hospital and urban wastewater in Brazil. ENVIRONMENTAL MONITORING AND ASSESSMENT, v. 192, n. 6, . (17/10723-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
ZAGUI, Guilherme Sgobbi. Evaluation of antibiotic multi-resistance and production of ESBL and carbapenemases in gram-negative bacilli of hospital and urban effluent. 2019. Master's Dissertation - Universidade de São Paulo (USP). Escola de Enfermagem de Ribeirão Preto (PCARP/BC) Ribeirão Preto.

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