Evaluation of vertical transmission of arboviruses in Ae. aegypti and Ae. albopictus (Diptera: Culicidae) in natural conditions

Abstract

In Brazil, the mosquito Aedes aegypti is considered the main vector of the dengue virus, chikungunya and Zika, being highly associated to humans and their habitations. Another mosquito - Aedes albopictus - is also widely distributed throughout the country and can not be neglected, it has great medical importance because is considered a potential vector of these three arboviruses. Worldwide, dengue, chikungunya and Zika viruses are transmitted to humans by the bite of infected females of these two species, which is called horizontal transmission. In addition, infected females can transfer arboviruses to part of their offspring, it is known as vertical transmission. The study of vertical transmission is of great epidemiological importance, it allows to understand the way in which the virus stays in nature in interepidemic periods. This work aims to verify the presence of vertical transmission of the dengue virus in natural populations of Ae. Aegypti and Ae. Albopictus and present a bibliographical survey about the presence of vertical transmission of the dengue, chikungunya and Zika viruses in these same species in the world. For this, eggs of both species were collected in Piqueri Municipal Park, East Zone of São Paulo, SP, with the use of ovitraps. The eggs were raised in laboratory conditions to the adult form. These adults were cryopreserved in freezer -80ºC for subsequent extraction of viral RNA and amplification by PCR technique. The bibliographic survey will be conducted through searches in the main online search databases. Articles will be read, evaluated and later separated by virus type and space unit.