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Determination and standardization of culture medium favorable for sperm incubation

Grant number: 17/03599-1
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): October 01, 2017
Effective date (End): April 30, 2019
Field of knowledge:Health Sciences - Medicine
Principal Investigator:Jorge Hallak
Grantee:Caroline Ranéa
Host Institution: Hospital das Clínicas da Faculdade de Medicina da USP (HCFMUSP). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil


Semen preparation techniques in Assisted Reproduction laboratory are used to improve the sperm quality, fecundity and pregnancy rates. Often these techniques uses culture media supplementation with nutrients to enrich them and increase sperm survival. The present study aims to determine which culture media and / or supplementation contribute to sperm quality, from a sperm functional point of view, after incubation. To understand these relationships, a prospective study will be carried out using seminal samples obtained from 50 infertile patients referred to the Androscience Laboratory. Initially, routine seminal analysis and samples are classified in four groups, in according to culture media and supplementation: Group I - HTF comum media + SSS (control); Group II - Sperm Rinse Media + Fructose + Carnitine; Group III - G-IVF Plus Media + Fructose + SSS; Froup IV - CSCM Media + HSA. All samples are submitted to viability and hyperactive evaluation, radical oxygen species levels and integrity DNA test pre and post-incubation at 37°C CO2% environment.We will use Student's t test and analysis of variance (ANOVA) to compare study variables. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
RANEA, CAROLINE; PARIZ, JULIANA RISSO; DREVET, JOEL R.; HALLAK, JORGE. perm motility in asthenozoospermic semen samples can be improved by incubation in a continuous single culture medium (CSCM (R). SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE, v. 68, n. 1, p. 25-35, . (17/03599-1, 18/26171-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
RANÉA, Caroline. Determination of favorable culture medium and sperm incubation period of asthenozoospermic seminal samples. 2020. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina (FM/SBD) São Paulo.

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