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Advances in sequential injection chromatography: two-dimensional chromatography and development of new stationary phases

Grant number: 17/08611-0
Support Opportunities:Scholarships in Brazil - Post-Doctoral
Effective date (Start): September 01, 2017
Effective date (End): August 31, 2019
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
Principal Investigator:Fábio Rodrigo Piovezani Rocha
Grantee:Mariana Roberto Gama Sato
Host Institution: Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
Associated research grant:11/23498-9 - Advanced flow systems for technological, agronomical and environmental analysis, AP.TEM


Sequential Injection Chromatography (SIC) utilizes low-pressure chromatographic columns coupled to sequential injection systems. Separation capacity in SIC can be increased with some analytical strategies, including the development of new, more selective stationary phases available as monolithic columns. The introduction of the monolithic stationary phases was an advance in the technology of chromatographic columns, as it presented advantages such as high permeability, which culminated in high mobile phase flows under lower backpressures; wide variability of precursors available for the preparation of new stationary phases, simple preparation processes, and high mechanical resistance. Hydrophilic Interaction Chromatography (HILIC) is a combination of the normal phase, reverse phase and ion chromatography separation modes because it employs highly hydrophilic stationary phases typical of those used in normal mode such as non-derivatized silica or that chemically modified with polar groups, such as amino, cyano, diol or polyols. In addition, HILIC applies polar organic solvents and their mixtures, commonly used as mobile phases for reversed phase, and is capable of separating the target compounds by ion chromatography, such as ionized or ionizabled polar ones. The development of multidimensional separation systems is an alternative in SIC by exploring chromatographic dimensions based on different separation mechanisms. When the sample is subjected to this process, separation and distribution of the analytes are inherently improved, increasing resolution. For this reason, stationary phases for use in HILIC mode are usual in combination with traditional stationary phases for reversed phase mode in two-dimensional orthogonal chromatographic separations, because the incompatibility of mobile phases is avoided. In addition, polar analytes, which exhibit low reverse phase separation performance, can be successfully separated on hydrophilic stationary phases for HILIC.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ACEVEDO, MARIA SOLEDAD M. S. F.; GAMA, MARIANA R.; BATISTA, ALEX D.; ROCHA, FABIO R. P.. Two-dimensional separation by sequential injection chromatography. Journal of Chromatography A, v. 1626, . (17/08611-0)
GAMA, MARIANA R.; ROCHA, FABIO R. P.. Solventless separation of underivatized biogenic amines by sequential injection chromatography. Microchemical Journal, v. 156, . (17/08611-0)
GAMA, MARIANA R.; ROCHA, FABIO R. P.; BOTTOLI, CARLA B. G.. Monoliths: Synthetic routes, functionalization and innovative analytical applications. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, v. 115, p. 39-51, . (17/08611-0)

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