In 2006, Shinya Yamanaka's group reported that somatic cells could be reprogrammed into pluripotent stem cells (induced pluripotent cells, iPSCs) by retroviral overexpression of four specific transcription factors: OCT4, C-MYC, SOX2 and KLF4 (OMSK). Since then, the establishment of iPS cell technology has been through a great development in the field of biology and medical science. Much effort has been invested in revealing the pathways leading to reactivation of the endogenous pluripotency apparatus in differentiated cells, and in identifying factors that regulate this process. Recent efforts focused on developing technologies to derive transgene-free iPS cells with the aim of obtaining cells that are safe, robust and reproducible. However many have investigated the reprogramming of mouse and human somatic cells into iPS cells, but few reports have emerged on successful reprogramming of differentiated somatic cell-derived induced pluripotency stem cells (iPS) in domestic species, for example, canine and swine. Indeed, both species are considered adequate models for human medicine. Therefore, this study aims to understand the underlying mechanisms that lead to canine induced pluripotency stem cells (ciPS) with two different reprogramming techniques: the use of integrative (lentiviral) or not (episomal) vectors; as well as to characterize the produced cells regarding their pluripotency. It is expected that the results obtained herein may elucidate the biotechnological potential of these cells to be further used in disease modeling.
News published in Agência FAPESP Newsletter about the scholarship: