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Clinical evaluation of different intracanal medications on the periapical levels of resolvins and lipoxin - new endodontic perspectives

Grant number: 16/26012-3
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): August 01, 2017
Effective date (End): December 31, 2018
Field of knowledge:Health Sciences - Dentistry - Endodontics
Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Marcia Carneiro Valera Garakis
Grantee:Bruna Jordão Motta Corazza
Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil


The aim of this study is to evaluate the effects of endodontic therapy with different root canal medications in disinfection and return of homeostasis by lipid mediators' quantification in the periapical fluid of teeth with primary endodontic infection. Thirty six single rooted teeth, with primary endodontic infection and apical periodontitis will be selected and randomly assigned into 3 groups (n=12): G1: saline + Ca(OH)2, G2: chlorhexidine, G3: chlorhexidine + Ca(OH)2. Microbial and endotoxic samples will be collected from the root canal, with sterile and apyrogenic paper point, to observe the microbial activity of remaining microorganisms in the root canal by colony-forming units (CFUs) and endotoxin quantification (EU/mL) by Limulus Amebocyte Lysate Assay - LAL, respectively. The intersticial fluid will be collected with sterile and apyrogenic paper points and used for lipid mediators (RvE1, RvD2, LxA4) quantification by Enzyme Linked ImmunonoSorbent Assay (ELISA). The root canals will be instrumented with reciprocating file (WaveOne Gold), a single reciprocating file selected in accord to diameter of root canal and the irrigating solution will be 2.5% NaOCl. After PQM the teeth will be filled with the intracanal medications according to the experimental groups. Fourteen days later, the canal will be re-instrumented and new collections will be performed. Results will be analyzed and submitted to statistical analysis (P <0.05). (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MARTINHO, FREDERICO C.; CORAZZA, BRUNA J. M.; KHOURY, RAYANA D.; OROZCO, ESTEBAN I. F.; TOIA, CASSIA C.; MACHADO, FELIPE P.; VALERA, MARCIA C.. Impact of N-acetylcysteine (NAC) and calcium hydroxide intracanal medications in primary endodontic infection: a randomized clinical trial. CLINICAL ORAL INVESTIGATIONS, v. 27, n. 2, p. 10-pg., . (16/26012-3, 18/01703-9)
CORAZZA, B. J. M.; MARTINHO, F. C.; KHOURY, R. D.; TOIA, C. C.; OROZCO, E. I. F.; PRADO, R. F.; MACHADO, F. P.; VALERA, M. C.. Clinical influence of calcium hydroxide and N-acetylcysteine on the levels of resolvins E1 and D2 in apical periodontitis. International Endodontic Journal, v. 54, n. 1, p. 61-73, . (16/26012-3, 18/01703-9)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
CORAZZA, Bruna Jordão Motta. Clinical evaluation of the effect of different intracanal medications on levels of periapical resolvins and lipoxins: new endodontic perspectives. 2018. Master's Dissertation - Universidade Estadual Paulista (Unesp). Instituto de Ciência e Tecnologia. São José dos Campos São José dos Campos.

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