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Identification of phosphorylation targets for Trichoderma reesei MAPK through phosphoproteomics during the production of cellulases.

Grant number: 17/07347-7
Support type:Scholarships in Brazil - Master
Effective date (Start): August 01, 2017
Effective date (End): July 31, 2018
Field of knowledge:Biological Sciences - Biochemistry - Molecular Biology
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:Roberto do Nascimento Silva
Grantee:Cláudia Batista Carraro
Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The increasing concern with the diminished fossil fuels sources and with all the environmental damage this kind of energy causes has led to the need of innovating the production of biofuels as a renewable and sustainable energy source. Since Brazil is the biggest sugarcane producer in the world and one of the firsts in bioethanol production, the country is responsible for the greatest generation of lignocellulosic residues from this kind of crop. Trichoderma reesei is a very efficient producer of lignocellulosic enzymes, thus it shows great industrial importance regarding to the degradation of the lignocellulosic material in sugar monomers capable of going through alcoholic fermentation. In order to optimize the degradation process so the whole bioethanol production can become financially viable, the complete characterization of MAPK pathways is needed, since these pathways are responsible for the transcriptional response to external stimuli and regulate the production of enzymes and metabolites inside the fungal cell. In this project, we aim to identify the direct and indirect targets for phosphorylation by MAPK, as well as the phosphorylation sites in transcription factors related to the regulation lignocellulosic enzymes, through phosphoproteomics, in T. reesei cultivated in glucose and sugarcane bagasse. (AU)

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Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
CARRARO, Cláudia Batista. Identification of phosphorylation targets for Trichoderma reesei MAPKs through phosphoproteomics during the production of cellulases. 2018. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC) Ribeirão Preto.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.