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Influence of the Cerebellar Histaminergic System on the motor and non-motor functions of mice

Grant number: 16/26071-0
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): July 01, 2017
Effective date (End): January 31, 2018
Field of knowledge:Humanities - Psychology - Physiological Psychology
Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Anna Carolyna Lepesteur Gianlorenço
Grantee:Evelyn Maria Guilherme
Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil


The cerebellum is an important structure which participates in motor processes such as gait and balance. Currently, studies have demonstrated its involvement in cognition, emotion and learning and memory processes. Even though the histaminergic system has an important role in the neuronal circuit within the cerebellum, its mode of action is not completely understood. The histamine modulation in different body systems occurs through four types os receptors: H1, H2, H3 and H4. The results about the cerebellar histaminergic system action in the motor performance are controversial. Therefore, the aim of this study is to investigate the effects of histamine and histaminergic antagonists microinjections via cerebellar vermis, investigating a possible interaction of this system within the cerebellum in the modulation of motor and non-motor functions. The four experiments will evaluate Histamine (experiment 1), histamine H1 receptors antagonist, Chlorpheniramine (experiment 2), H2 receptors antagonist, Ranitidine (experiment 3) and H3 receptors antagonist, Thioperamide (experiment 4) effects on locomotor activity and anxiety in mice exposed to open field. In order to achieve this purpose, a 7 mm long cannula will be implanted in the cerebellar vermis through stereotactic surgery performed in male mice (Swiss Albino) weighting between 25 and 35 g. The open-field behavior test will be executed three days after surgery. The experiments will be recorded to quantify the amount of crossings (number of quadrants traversed), time spent in the central area of the arena, freezing time and number of rearings. The histologic analysis will confirm if the injection occurred appropriately in the cerebellar vermis. Statistical analysis will include the homogeneity test and analysis of variance (ANOVA), followed by Duncan's post-hoc test. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GUILHERME, EVELYN M.; SILVA-MARQUES, BRUNA; FERNANDES, CARLOS EDUARDO M.; RUSSO, THIAGO L.; MATTIOLI, ROSANA; GIANLORENCO, ANNA C.. Intracerebellar microinjection of histaminergic compounds on locomotor and exploratory behaviors in mice. Neuroscience Letters, v. 687, p. 10-15, . (16/26071-0, 14/24774-8)

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