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Proteomic analysis of the acquired enamel pellicle and saliva in patients with head and neck cancer that are submitted radiotherapy

Grant number: 17/05031-2
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): July 01, 2017
Effective date (End): April 30, 2021
Field of knowledge:Health Sciences - Dentistry - Social and Preventive Dentistry
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:Marília Afonso Rabelo Buzalaf
Grantee:Talita Mendes Oliveira Ventura
Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
Associated scholarship(s):19/16815-0 - Protective role of acquired enamel pellicle formed for different times and of Statherin peptide against initial dental erosion: evaluation using a new hand-held optical reflectometer, BE.EP.DR


Cancer is one of the main causes of mortality in all world, and is currently a major public health problem. The acquired pellicle (PA) is a tegument formed in vivo as a result of the selective adsorption of salivary proteins on a surface of the tooth, which still contains glycoproteins and lipids. The presence of proteins in PA forms a protective interface on a tooth surface, participating in all the interfacial events that occur in the oral cavity. It is an organic film, bacterial-free, which covers the dental tissues. Studies that focus on characterization and not impact protector of the acquired pellicle formed on an enamel surface. However, little is known about how composition acquired pellicle in cancer patients with head and neck cancer undergoing radiotherapy treatment, which interferes with the amount and quality of saliva. Based on this, the objective of this study is to evaluate the protein profile of the acquired pellicle and saliva in cancer patients diagnosed with head and neck cancer and submitted to radiotherapy treatment. Twenty subjects, aged over 18 years, 10 participants without head and neck cancer, non-smokers, with good general and oral health and 10 other diagnosed with head and neck cancer submitted to radiotherapy. The acquired pellicle will be formed in the morning, for 120 minutes, after prophylaxis with pumice. Pellicles and salivas formed in different treatment periods (before, during and after treatment with radiotherapy) are collected separately for analysis using 3% citric acid-soaked paper. As samples are processed for nLC-ESI-MS / MS analysis. MS / MS spectra are obtained from human protein databases (SWISS-PROT). Free marker quantification will be performed using the PLGS software. (AU)

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
VENTURA, TALITA MENDES OLIVEIRA; RIBEIRO, NATHALIA REGINA; TAIRA, EVEN AKEMI; DE LIMA LEITE, ALINE; DIONIZIO, ALINE; RUBIRA, CASSIA MARIA FISCHER; DA SILVA SANTOS, PAULO SERGIO; BUZALAF, MARILIA AFONSO RABELO. Radiotherapy changes the salivary proteome in head and neck cancer patients: evaluation before, during, and after treatment. CLINICAL ORAL INVESTIGATIONS, MAY 2021. Web of Science Citations: 0.
PELA, VINICIUS T.; LUNARDELLI, JOAO G. Q.; VENTURA, TALITA M. O.; CAMILOTI, GABRIEL D.; BAUMANN, TOMMY; CARVALHO, THIAGO S.; LUSSI, ADRIAN; BUZALAF, MARILIA A. R. Proteomic profiles of the acquired enamel pellicle formed in vitro, in situ, or in vivo. European Journal of Oral Sciences, v. 128, n. 6 NOV 2020. Web of Science Citations: 0.
VINÍCIUS TAIOQUI PELÁ; TALITA MENDES OLIVEIRA VENTURA; MARÍLIA AFONSO RABELO BUZALAF. Optimizing the formation of the acquired enamel pellicle in vitro for proteomic analysis. Journal of Applied Oral Science, v. 28, p. -, 2020. Web of Science Citations: 0.
TALITA MENDES DA SILVA VENTURA; NATHALIA REGINA RIBEIRO; ALINE SALGADO DIONIZIO; ISABELA TOMAZINI SABINO; MARÍLIA AFONSO RABELO BUZALAF. Standardization of a protocol for shotgun proteomic analysis of saliva. Journal of Applied Oral Science, v. 26, p. -, 2018. Web of Science Citations: 0.

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