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Effect of a fluconazole carrier nanosystem on the extracellular matrix composition and structure of Candida biofilms

Grant number: 17/08980-5
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): July 01, 2017
Effective date (End): June 30, 2018
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal Investigator:Douglas Roberto Monteiro
Grantee:Raphael Fernando Barion Ferraresse
Host Institution: Faculdade de Ciências da Saúde (FCSA). Universidade do Oeste Paulista (UNOESTE). Presidente Prudente , SP, Brazil


The aim of this study is to synthesize a new fluconazole (FLZ) carrier nanosystem based on magnetic iron oxide nanoparticles (MNPs) and chitosan (CTS), as well as to evaluate the effect of this nanosystem on the extracellular matrix composition and structure of Candida albicans and Candida glabrata biofilms. The nanosystem will be prepared by loading of FLZ on MNPs coated with CTS and characterized by X-ray diffraction, infrared spectroscopy, thermogravimetric analysis and transmission electron microscopy. The minimum inhibitory concentration of the FLZ-CTS-MNP nanosystem capable of inhibiting the strains in planktonic state will be determined according to the microdilution method. Biofilms of the two species will be formed (for 24 hours) into wells of 96-well plates and treated twice a day, during 2 days, with different concentrations of the nanosystem. After, the effect of the nanosystem will be evaluated on the extracellular matrix composition (proteins and carbohydrates) and structure of the biofilms (using scanning electron microscopy). Appropriate controls will be included in all analyzes. Data will be analyzed to employ the most appropriate statistical test. P < .05 will be considered statistically significant. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE LIMA, TAYNARA M. T.; ARIAS, LAIS S.; AFANACI, LETICIA F.; FERRARESSE, RAPHAEL F. B.; NETO, FRANCISCO N.; DE LIMA, BRUNO H. R.; STRAIOTO, FABIANA G.; DE CAMARGO, EMERSON R.; PESSAN, JULIANO P.; MONTEIRO, DOUGLAS R.. Assembly and antifungal effect of a new fluconazole-carrier nanosystem. FUTURE MICROBIOLOGY, v. 15, n. 4, p. 273-286, . (17/24416-2, 17/08976-8, 17/08980-5)

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