Canine adipose-derived mesenchymal stem cells viability following exposure to different molecular weights of hyaluronic acid

Abstract

The transplantation of mesenchymal stem cells (MSC) is becoming a promising treatment for osteoarthritis (OA), as well the intra-articular application of hyaluronic acid (HA), that has been shown effective in pain reduction and supporting the homeostasis in the articular environment. Moreover, the combination of HA with MSC has been demonstrated as a therapeutic potential for OA treatment. However, considering the different molecular weights of HA available for therapy, it is important to study the effects of these different molecular weights in contact with MSC. It will be evaluated canine adipose derived mesenchymal stem cells viability following exposure to high and low molecular weights of hyaluronic acid. Cells were harvested and assessed for viability using trypan blue exclusion. (AU)