One of the major challenges of biotechnology is the development of biological processes that reduce the negative impacts on the environment caused by the burning of fossil fuels. In this context, biofuels have received attention in recent decades. Trichoderma reesei and Aspergillus niger are among the main producers of lignocellulolytic enzymes, which are used in the conversion of lignocellulosic raw materials to fermentable sugars. However, the mechanism of biomass degradation between these fungi seems to be different. Although there are advances in research related to the activation and production of these enzymes, little is known about the transport systems for the sugar uptake and the influence of these transporters on the degradation of the biomass. The knowledge about fungal transport is essential for understanding the molecular mechanisms involved in the process of lignocellulosic biomass deconstruction for the development of new technologies that aim to optimize the process of obtaining bioethanol and reduce the cost of biofuel. Therefore, the project aims to identify and classify the sugar transporters involved in the process of lignocellulosic biomass deconstruction in these fungi, using tools for in silico and in vivo analysis of its membrane-associated proteome, through mass spectrometric measurements and proteomics data analysis. Subsequently, the characterization of these identified transporters will provide information to describe the differences in the biomass degradation mechanism of each fungus and why this occurs.
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