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Comparison between the antimicrobial effects of Photodynamic Antimicrobial Therapy mediated by curcumin and chlorhexidine on microcosms of dentin caries lesions

Grant number: 16/24962-4
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): June 01, 2017
Effective date (End): December 31, 2017
Field of knowledge:Health Sciences - Dentistry - Pediatric Dentistry
Principal Investigator:Thiago Cruvinel da Silva
Grantee:Eliézer Gutierres
Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil


This study aims to evaluate the immediate and late effect of Antimicrobial Photodynamic Therapy (aPDT) and chlorhexidine on specific microbial groups from dentin caries microcosms. Dentin samples will be collected from cavitated lesions of ten 7-11 year-old children to grow biofilms on bovine dentin specimens for 5 days. Subsequently, biofilms will be treated either by chlorhexidine digluconate solution or by the association between curcumin and a blue LED light source (420 nm). The viability of specific microbial groups will be determined by counts of colony forming units of total microorganisms (Tryptic Soy Blood Agar), total streptococci (Mitis Salivarius Agar), mutans streptococci (Mitis Salivarius Agar plus 20% sucrose and 0.2% bacitracin), and lactobacilli (Rogosa Agar plus 0.13% glacial acetic acid). The method of enzymatic spectrophotometry will be performed to evaluate the concentration of L-lactate in the culture media. Statistical analysis will be based on the normality and homogeneity of the data. P values <0.05 will be considered significant. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CHAVES LAMARQUE, GIULIANA CAMPOS; CUSICANQUI MENDEZ, DANIELA ALEJANDRA; GUTIERREZ, ELIEZER; DIONISIO, EVANDRO JOSE; ANDRADE MOREIRA MACHADO, MARIA APARECIDA; OLIVEIRA, THAIS MARCHINI; RIOS, DANIELA; CRUVINEL, THIAGO. Could chlorhexidine be an adequate positive control for antimicrobial photodynamic therapy in- in vitro studies?. Photodiagnosis and Photodynamic Therapy, v. 25, p. 58-62, . (14/10897-0, 16/24962-4)

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