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Molecular interactions of the Golgi Reassembly and Stacking Protein (GRASP) form Saccharomyces cerevisiae

Grant number: 16/23863-2
Support Opportunities:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): June 01, 2017
Effective date (End): June 30, 2021
Field of knowledge:Biological Sciences - Biophysics - Molecular Biophysics
Principal Investigator:Antonio José da Costa Filho
Grantee:Natália Aparecida Fontana
Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated scholarship(s):18/18717-2 - Structural studies of the Golgi reassembly and stacking protein from S. cerevisiae and its golgin, BE.EP.DD


Golgi Reassembly and Stacking Proteins (GRASPs) have been involved in a variety of functions including non-conventional secretion and organization of the structure of the Golgi apparatus. However, their precise role in each of those problems still deserves more detailed structural and functional studies at a molecular level. It is within this context that our project finds its main goal: the production of data that will allow understanding the structure-function correlation of the GRASP from S. cerevisiae (Grh1). The use of model organisms, such as S. cerevisiae, is strategic to obtain comprehensive information on the cell processes that GRASPs are taking part. We have the chance of generating knowledge in two biologically-relevant problems: (1) although GRASPs have been implicated in a broad set of functions, a definitive understanding of their functions in several organisms is far from complete; (2) detection and characterization of intrinsically disordered regions in proteins, which presents great potential of impact over the understanding of the role of intrinsically disordered proteins in cell processes. Thus, in the present project we aim at obtaining experimental data on the molecular interactions taking place during the Grh1 (GRASP from S. cerevisiae) functional cycle. To do so we will make use of a multidisciplinary and multitechnique approach involving methods of molecular biology and biochemistry as well as molecular biophysical tools, such as circular dichroism and electron magnetic resonance. (AU)

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MENDES, LUIS F. S.; FONTANA, NATALIA A.; OLIVEIRA, CAROLINA G.; FREIRE, MARJORIE C. L. C.; LOPES, JOSE L. S.; MELO, FERNANDO A.; COSTA-FILHO, ANTONIO J.. The GRASP domain in golgi reassembly and stacking proteins: differences and similarities between lower and higher Eukaryotes. FEBS Journal, v. 286, n. 17, p. 3340-3358, . (17/24669-8, 12/20367-3, 15/50366-7, 09/53989-4, 16/23863-2)
MENDES, LUIS F. S.; FONTANA, NATALIA A.; REDDY, S. THIRUPATHI; UVERSKY, VLADIMIR N.; COSTA-FILHO, ANTONIO J.. The exquisite structural biophysics of the Golgi Reassembly and Stacking Proteins. International Journal of Biological Macromolecules, v. 164, p. 3632-3644, . (12/20367-3, 15/50366-7, 16/23863-2, 17/24669-8, 17/12146-0)
THIRUPATHI REDDY, S.; SANTOS MENDES, LUIS FELIPE; FONTANA, NATALIA APARECIDA; COSTA-FILHO, ANTONIO JOSE. Exploring structural aspects of the human Golgi matrix protein GRASP55 in solution. International Journal of Biological Macromolecules, v. 135, p. 481-489, . (16/23863-2, 17/12146-0, 17/24669-8, 15/16812-0, 15/50366-7, 12/20367-3)
FONTANA, N. A.; FONSECA-MALDONADO, R.; MENDES, L. F. S.; MELEIRO, L. P.; COSTA-FILHO, A. J.. The yeast GRASP Grh1 displays a high polypeptide backbone mobility along with an amyloidogenic behavior. SCIENTIFIC REPORTS, v. 8, . (15/18390-5, 16/09676-5, 15/50366-7, 12/13309-7, 12/20367-3, 16/23863-2)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
FONTANA, Natália Aparecida. Golgi Reassembly and Stacking Protein: biophysical properties and their functional implications. 2021. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (PCARP/BC) Ribeirão Preto.

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