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Comparative genomic hybridization, microdissection and chromosome painting in Hepsetus akawo (Characiformes, Hepsetidae)

Grant number: 17/08471-3
Support Opportunities:Scholarships abroad - Research Internship - Scientific Initiation
Effective date (Start): July 22, 2017
Effective date (End): August 21, 2017
Field of knowledge:Biological Sciences - Genetics - Animal Genetics
Principal Investigator:Marcelo de Bello Cioffi
Grantee:Pedro Cristovão Carvalho
Supervisor: Thomas Liehr
Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
Research place: Friedrich Schiller University Jena, Germany  
Associated to the scholarship:16/17556-0 - Evolutionary relationships of the Erythrinidae family (Teleostei, Characiformes). IV: comparative cytogenetics in Hepsetidae, BP.IC


The student has developed 6 months of the project entitled: "Evolutionary relationships of the Erythrinidae family (Teleostei, Characiformes). IV. Comparative cytogenetics in Hepsetidae". Up to now, great effort was depicted to sample these fishes in the Osun river (Nigeria) and analyze them by conventional (Giemsa and C-banding) and molecular (chromosomal mapping of multigene families, Transposable elements and several microsatellite repeats) cytogenetic methods, providing important and unprecedented data for the African Hepsetidae family. Thus, taking into account the good progress of the project and the student's involvement in the experiments, we aim to deepen into multipronged and modern molecular cytogenetic approaches, including Comparative Genomic Hybridization (CGH), microdissection of sex chromosomes and chromosome painting experiments using the technique of multicolor FISH (m-FISH) in H. akawo. We will map the gDNA and the sex chromosomes of distinct Erythrinidae specimens, taking into account to test possible relationships among them. The experiments will be performed in the laboratory of Molekulare Zytogenetik (Universitätsklinikum Jena - Germany), headed by Prof. Dr. Thomas Liehr, researcher of global significance in chromosome microdissection and FISH. The data obtained will make it possible to elucidate the fundamental aspects of the evolution of sex chromosomes and karyotype evolution among fishes, considering the complex investigation of these chromosomes between closely related families. Finally, the results also make important contributions to the understanding of the evolution of sex chromosomes in vertebrates as a whole. In addition, besides the expected scientific advances, this proposal will provide a unique opportunity for the scientific training of the student, by interaction with renowned group of German research and learning new study methodologies currently employed in Dr. Liehr laboratory. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CARVALHO, PEDRO C.; DE OLIVEIRA, EZEQUIEL A.; BERTOLLO, LUIZ A. C.; YANO, CASSIA F.; OLIVEIRA, CLAUDIO; DECRU, EVA; JEGEDE, OLADELE I.; HATANAKA, TERUMI; LIEHR, THOMAS; AL-RIKABI, AHMED B. H.; et al. First Chromosomal Analysis in Hepsetidae (Actinopterygii, Characiformes): Insights into Relationship between African and Neotropical Fish Groups. FRONTIERS IN GENETICS, v. 8, . (16/21411-7, 16/17556-0, 14/26508-3, 17/08471-3)

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