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Study of chitosan and hydrophilic derivatives by time domain NMR

Grant number: 16/20970-2
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): May 01, 2017
Effective date (End): April 30, 2021
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
Principal researcher:Luiz Alberto Colnago
Grantee:William Marcondes Facchinatto
Home Institution: Embrapa Instrumentação Agropecuária. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). Ministério da Agricultura, Pecuária e Abastecimento (Brasil). São Carlos , SP, Brazil
Associated scholarship(s):19/11135-0 - Clotrimazole-loaded N-(2-hydroxy)-propyl-3-trimethylammonium, O-Palmitoyl chitosan micelles: preparation, characterization and in vitro evaluation against Candida species, BE.EP.DR


Chitosan (Ch) is a linear copolymer obtained from natural source, frequently studied for biotechnological applications, but it has limited solubility over pH < pKa values. The derivatization of Ch chains, on the other hand, with hydrophilic chains of poly(ethylene glycol) or by -N(CH3)+ glicidiltrimetilamônio substituent insertion in the GlcN units, not only increases the solubility for Ch chains at neutral and alkaline pH, but also increases the bioavailability in vivo, biocompatibility, immunogenicity, antimicrobial activity and mucoadhesiveness, such properties especially useful for biomedical applications, particularly for bioactive delivery systems. The performance of Ch-based and derivatives biomaterial systems are often affected by structural parameters such as the average degree and distribution of block or random acetamido groups (GlcNAc units) and the average molar mass of polymer chains. Therefore, currently there are different techniques and analytical methods to characterize the influence of these parameters on the properties of Ch and derivatives in solution, as well as the ability of interaction of Ch chains with metal ions for biological and environmental applications. However, recently it was found that the relaxation processes (T1 and T2) of the solvent in Ch solutions, determined by Time Domain NMR (TD-NMR) is directly related to the colloidal properties of Ch. In this sense, this work aims to explore a systemic relationship between structure / colloid property and interaction with paramagnetic ions with Ch produced from USAD process with different structural and physicochemical characteristics, as well as for derivatives N-methoxypoly(ethylene glycol)-graft-chitosan (PCh) and N-(2-hydroxy) propyl-3-trimethylammonium chitosan (QCh) by simultaneous relaxation measurements in CWFP and CP-CWFP methods in order to correlate the values of T1 and T2 with the thermal stability, crystallinity, pH variation, viscosity, zeta potential and average hydrodynamic diameter distribution of macromolecules in solution. (AU)

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Scientific publications (7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FACCHINATTO, WILLIAM MARCONDES; GALANTE, JOANA; MESQUITA, LETICIA; SILVA, DANIELLA SOUZA; DOS SANTOS, DANILO MARTINS; MORAES, TIAGO BUENO; CAMPANA-FILHO, SERGIO PAULO; COLNAGO, LUIZ ALBERTO; SARMENTO, BRUNO; DAS NEVES, JOSE. Clotrimazole-loaded N -(2-hydroxy)-propyl-3-trimethylammonium, O -palmitoyl chitosan nanoparticles for topical treatment of vulvovaginal candidiasis. Acta Biomaterialia, v. 125, p. 312-321, . (16/20970-2, 19/13656-8, 17/20973-4, 19/11135-0)
FACCHINATTO, WILLIAM MARCONDES; DOS SANTOS, DANILO MARTINS; BUKZEM, ANDREA DE LACERDA; MORAES, TIAGO BUENO; HABITZREUTER, FILIPE; DE AZEVEDO, EDUARDO RIBEIRO; COLNAGO, LUIZ ALBERTO; CAMPANA-FILHO, SERGIO PAULO. Insight into morphological, physicochemical and spectroscopic properties of beta-chitin nanocrystalline structures. Carbohydrate Polymers, v. 273, . (19/13656-8, 17/20973-4, 16/20970-2)
SILVA, DANIELLA SOUZA; FACCHINATTO, WILLIAM MARCONDES; SANTOS, DANILO MARTINS DOS; BONI, FERNANDA ISADORA; VALDES, TALITA ALVARENGA; LEITAO, ANDREI; GREMIAO, MARIA PALMIRA DAFLON; COLNAGO, LUIZ ALBERTO; CAMPANA-FILHO, SERGIO PAULO; RIBEIRO, SIDNEY JOSE LIMA. N-(2-hydroxy)-propyl-3-trimethylammonium, O-palmitoyl chitosan: Synthesis, physicochemical and biological properties. International Journal of Biological Macromolecules, v. 178, p. 558-568, . (16/20970-2, 19/13656-8, 17/20973-4)
CARDOZO, FLAVIO AUGUSTO; FACCHINATTO, WILLIAM MARCONDES; COLNAGO, LUIZ ALBERTO; CAMPANA-FILHO, SERGIO PAULO; PESSOA, ADALBERTO. Bioproduction of N-acetyl-glucosamine from colloidal alpha-chitin using an enzyme cocktail produced by Aeromonas caviae CHZ306. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, v. 35, n. 8, . (16/20970-2, 12/16824-0, 13/18773-6)
FACCHINATTO, WILLIAM MARCONDES; FIAMINGO, ANDERSON; DOS SANTOS, DANILO MARTINS; CAMPANA-FILHO, SERGIO PAULO. Characterization and physical-chemistry of methoxypoly (ethylene glycol)-g-chitosan. International Journal of Biological Macromolecules, v. 124, p. 828-837, . (17/20973-4, 16/09720-4, 16/20970-2, 10/02526-1)
VILLAS-BOAS, FLAVIA; FACCHINATTO, WILLIAM MARCONDES; COLNAGO, LUIZ ALBERTO; VOLANTI, DIOGO PASCHOALINI; LANDI FRANCO, CELIA MARIA. Effect of amylolysis on the formation, the molecular, crystalline and thermal characteristics and the digestibility of retrograded starches. International Journal of Biological Macromolecules, v. 163, p. 1333-1343, . (16/20970-2, 17/19521-1)
FACCHINATTO, WILLIAM MARCONDES; DOS SANTOS, DANILO MARTINS; FIAMINGO, ANDERSON; BERNARDES-FILHO, RUBENS; CAMPANA-FILHO, SERGIO PAULO; DE AZEVEDO, EDUARDO RIBEIRO; COLNAGO, LUIZ ALBERTO. Evaluation of chitosan crystallinity: A high-resolution solid-state NMR spectroscopy approach. Carbohydrate Polymers, v. 250, . (17/24465-3, 16/20970-2, 17/20973-4, 16/09720-4, 19/13656-8)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
FACCHINATTO, William Marcondes. Physicochemical studies of chemically modified chitosans through Solid-State Nuclear Magnetic Resonance: from properties to applications. 2021. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Química de São Carlos (IQSC/BT) São Carlos.

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