Analysis of clorina E6 effects in gliosarcoma treatment: in vitro study

Abstract

Gliosarcoma is a highly agressive malignant neoplasm that affects the Central Nervous System and accounts for 2% of all brain tumors. Despite the advances in conventional treatment techniques, which consist of surgical removal of the lesion, followed by radiotherapy and chemotherapy as secondary treatments, the prognosis of patients with CNS tumors continues to be unfavorable, making it necessary to develop alternative treatments. Photodynamic therapy (PDT) is a therapeutic modality used in the treatment of several malignant neoplasms. It consists in the combined action of a photosensitive compound (PS), light at wavelength appropriate to that compound and molecular oxygen, generating reactive oxygen species that lead tumor cells to death by necrosis or apoptosis. The objective of this work is to evaluate the cellular viability of 9L / LacZ gliosarcoma cells, submitted to PDT with chlorine e6, analyzing the potential of the therapy for the treatment of this type of malignant neoplasm and observing its action in vitro. The PS Fotoenticine® will be used at concentrations of 200 ¼g / ml to 6.25 ¼g / ml, in serial 1: 2 dilution, the light source will be a LED-based device (Biopdi / Irrad-Led 660) consisting of 54 LEDs, with 70mW of power in the 660nm region. For the analysis of the therapy action, the internalizatioin of PS by means of fluorescence microscopy and cell viability by trypan blue exclusion, MTT (mitochondrial activity) and morphological analysis under optical microscope (Leica DM 2500). (AU)