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Effects of interval training high intensity across the fluoride treatment in mice susceptible and resistant fluorosis

Grant number: 17/03703-3
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): May 01, 2017
Effective date (End): December 31, 2018
Field of knowledge:Health Sciences - Dentistry - Social and Preventive Dentistry
Principal Investigator:Marília Afonso Rabelo Buzalaf
Grantee:Fabielle Sales Raymundo
Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil


Fluoride (F) is extremely important for public health due to its properties to prevent or reverse caries in individuals of all ages. It can pose risks if consumed or applied indiscriminately or inappropriately. The main side effect of excessive use of fluoride is dental fluorosis. After absorption, F enters the blood stream and is distributed to the whole organism. It is stored mainly in calcified tissues. Its excretion occurs essentially via the kidney. Several molecular mechanisms involving F metabolism are still unknown. In the last decade, many studies have reported the influence of genetic factors in the susceptibility to dental fluorosis. Interesting studies employed mouse strains that have very different responses to the effects F. Thus, there is evidence that resistance or susceptibility to fluorosis seem to be influenced by genetic factors as well as by the interaction with the environment, such as physical activity. The current proposal is to analyze and compare parameters related to insulin resistance, as well as the fluoride retention and metabolism in mice susceptible or resistant to fluorosis, in response to changes in exposure F, with and without exercise. Therefore, 90 mice of A/J (n = 45) and 129P J (n = 45) strains, susceptible and resistant to fluorosis, respectively, will be obtained. The animals from each strain will be divided into 3 groups, according to the treatment (n=15/group), as follows: a) no exposure to F, no physical exercise; b) drinking water containing 50 ppm F (as NaF) for 56 days and no physical exercise; c) drinking water containing 50 ppm F (as NaF) for 56 days plus physical exercise (daily running for 5 days/week on a treadmill for 60 minutes at high intensity). After the experimental period, the animals will be submitted to euthanasia for samples collection and analysis. F will be analyzed in plasma, femur and liver, besides plasma glucose and insulin levels analysis. After being checked for normality and homogeneity, data will be submitted to appropriate statistic tests (p<0.05). (AU)

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