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In vivo characterization of the regulatory activity of Bacillus subtilis purine riboswitches

Grant number: 17/02594-6
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): April 01, 2017
Effective date (End): March 31, 2018
Field of knowledge:Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
Principal researcher:Danielle Biscaro Pedrolli
Grantee:Nathan Vinícius Ribeiro
Home Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
Associated research grant:14/17564-7 - Reprogramming the purine metabolism of Bacillus subtilis employing sRNA-technology, AP.JP

Abstract

In the first report related to the JP project, the regulatory activity of the Bacillus subtilis purine riboswitches were characterized in vitro. sRNAs designed to target these riboswitches were also assayed in vitro to access their functionality. Next, sRNAs functionality will be assayed in vivo using B. subtilis. Therefore, it is necessary to characterize the riboswitches in vivo first. (AU)

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