Effects of sugar consumption on the metabolic profile and prostatic morphophysiology in the male rats offspring submitted to gestational diabetes

Abstract

The increase in the consumption of "added sugar" has been associated with the epidemic of cases of diabetes mellitus (DM) observed worldwide. Similarly, the cases of gestational diabetes (GD) also have increased over the last decades. Although the negative effects of diabetes on various organs and systems are well known, the impact of DG, particularly on the offspring are still little known. Epidemiological and experimental data show that individuals born to diabetic mothers are more susceptible to develop obesity, diabetes and cardiovascular disease in adulthood. Recently, the negative impacts of DG on the reproductive capability of the offspring were also highlighted. Considering the increase in sugar consumption, especially among children and adolescents due to the high consumption of soft drinks, this project aims to assess whether offspring born to mothers rats with induced gestational diabetes are more susceptible to postnatal exposure to sugar consumption compared to rats born to normal pregnancy. In addition, considering data from our group demonstrated the negative effect of DG on the development and prostate maturation in offspring also will be assessed the impacts of sugar consumption on prostate morphophysiology after sugar consumption in these animals. It will be used to male offspring of Sprague Dawley rats submitted or not to DG induced by administration of streptozotocin (STZ, 30mg / kg body) in pregnant rats on the 10th gestational day (GD). The offspring will be divided into four groups: control (CTR): Mice born to mothers who had normal pregnancy and fed food and water ad libitum; Control + sucrose (CTR + SAC): Mice born to mothers who had normal pregnancy and who consumed sucrose solution (10% diluted in water) from postnatal day 21 (DPN21 weaning); Gestational diabetes (GD): Mice born to mothers with DG and fed food and water ad libitum; Gestational Diabetes + sucrose (DG + SAC): Mice born to mothers with DG and who consumed sucrose solution (10% diluted in water) from the DPN 21. DPN 90, the animals are weighed, euthanized and blood collected for metabolic and hormonal analysis, and the ventral prostate collected for morphological, immunohistochemical and biochemical analyzes. These results will help to understand the effects of DG on metabolic and prostate parameters of exposed offspring postnatal sugar consumption. This project falls under the research responsible line that, besides the effects of gestational diabetes on the different parameters offspring in the offspring also assesses the effects of fetal programming induced by gestational protein restriction on the development and aging of offspring, with special emphasis on prostatic morphophysiology. (AU)