The use of probiotics, microorganisms safe for human consumption and with large beneficial effects, has shown promise in treating periodontal disease (PD). However the mechanisms by which these agents can act in PD are still unclear. This project will evaluate the effect probiotics application on immune response of gingival epithelial cells challenged with Porphyromonas gingivalis and in the control of experimental PD. The effect of probiotic bacteria of the genus Bifidobacterium sp. and Lactobacillus sp. will be determined in a co-culture test of gingival epithelial cells OBA-9 challenged with P. gingivalis strains, W83 and ATCC 33277, followed by analysis of the ability of adhesion and invasion, wound healing and viability of gingival epithelial cells. Strains or combinations of probiotics that show the most promising results in these preliminary analyzes will be evaluated for their ability to interfere with the OBA-9 cell apoptosis process in co-culture with P. gingivalis and for their ability to modulate the immune response in these cells by flow cytometric analysis, gene expression (RT-PCR), Western blotting and quantitation of beta-defensins and cytokines by ELISA. After these assessments, the effect of probiotic strain or association most promising to control the PD will be determined using a experimental periodontitis model in mice induced by P. gingivalis. In this in vivo model will evaluate the action of probiotics in the development and treatment of PD by the analysis of alveolar bone loss and the characterization of Th1, Th2, Th17 and Treg cells subpopulations present in periodontal tissues and involved in the immune response in PD.
News published in Agência FAPESP Newsletter about the scholarship: