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The slow myoblast cell culture as a model to understand the myogenesis and growth of slow-twitch muscle

Grant number: 16/19683-9
Support Opportunities:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): March 01, 2017
Effective date (End): August 31, 2017
Field of knowledge:Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
Principal Investigator:Maeli Dal Pai
Grantee:Bruno Oliveira da Silva Duran
Supervisor: Daniel Garcia de La Serrana
Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
Research place: University of St Andrews, Scotland  
Associated to the scholarship:15/03234-8 - The pacu (Piaractus mesopotamicus) myoblast cell culture as a model to understand muscle growth regulation in Ostariophysi superorder, BP.DR


Skeletal muscle is the most abundant tissue in teleost fish, representing up to 65% of the total body mass. Skeletal muscle fibres are broadly classified as fast (fast-twitch muscle) and slow (slow-twitch muscle), which are found in separate compartments and have different contractile and metabolic properties. A class of small noncoding RNAs, known as microRNAs, plays an important post-transcriptional regulation of genes, controlling several aspects of muscle development. The in vitro fish myoblast cell culture has demonstrated a very useful tool to study muscle growth, development and regulation, since it recapitulates the steps of myogenesis: myoblast commitment, proliferation, fusion and myotube formation. In addition, the myoblast cell cultures can be manipulated to evaluate the role of different factors in the regulation of skeletal muscle myogenesis and growth. The compartmentalization of skeletal muscle in fish allows the establishment of individual myoblast cell cultures from fast and slow muscle, so that the physiological differences between these tissues could be better studied in such systems. However, the myoblast cell culture has been only developed for fast skeletal muscle, while a similar model for slow muscle remains to be established and explored. In this work we propose to develop a slow muscle cell culture in order to overcome the gap in knowledge about slow muscle development in comparison with fast skeletal muscle. The comparative study of slow and fast muscle cell culture, especially the role of muscle-specific microRNAs, will be useful to investigate differences between the molecular pathways that control the myogenic, metabolic, contractile and physiologic properties in the both muscle types. Our work will contribute to better understand the myogenesis regulation in fish, allowing the development of a theoretic framework that may help to improve muscle growth. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DA SILVA DURAN, BRUNO OLIVEIRA; DAL-PAI-SILVA, MAELI; GARCIA DE LA SERRANA, DANIEL. Rainbow trout slow myoblast cell culture as a model to study slow skeletal muscle, and the characterization of mir-133 and mir-499 families as a case study. Journal of Experimental Biology, v. 223, n. 2, . (16/19683-9, 15/03234-8, 16/05009-4)

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